Interactions of the complement system with the surface and endotoxic lipopolysaccharide of Veillonella alcalescens.
Interactions of the complement system with the surface and endotoxic lipopolysaccharide of Veillonella alcalescens.
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DOI：
10.1084/jem.125.5.767
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发表时间：
1967-05-01
期刊：
影响因子：
--
通讯作者：
Mergenhagen SE
中科院分区：
文献类型：
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作者：
Bladen HA;Gewurz H;Mergenhagen SE
电镜观察表明，内毒素脂多糖（LPS）不仅对产碱弧菌的细胞表面产生损伤，而且与新鲜豚鼠血清反应后，产碱弧菌的细胞表面也产生损伤。这些病变的直径约为90 A，并且在与血清孵育后来自LPS制剂的两个特征性结构实体上观察到。许多抑制剂、灭活剂和影响溶血C'活性的反应条件的使用表明，这些病变是由C'系统介导的。伴随病变形成，C'被固定;对经典C' 3活性的影响是显著的。得出结论，内毒素LPS，包含在细菌细胞的外三层膜，是C'酶的底物。提示内毒素的某些生物活性可能来源于其对C'系统的影响。
Electron microscopic studies demonstrated that lesions were produced on the endotoxic lipopolysaccharide (LPS) as well as on the cell surface of V. alcalescens after reaction with fresh guinea pig serum. These lesions were approximately 90 A in diameter, and were seen on two characteristic structural entities derived from LPS preparations after incubation with serum. The use of numerous inhibitors, inactivators, and reaction conditions affecting hemolytic C' activity revealed that these lesions were mediated by the C' system. Concomitant with lesion formation, C' was fixed; the effect on classical C'3 activity was pronounced. It is concluded that endotoxic LPS, as contained in the outer three-layered membrane of the bacterial cell, is a substrate for the C' enzymes. It is suggested that certain biological activities of endotoxin may derive from its effects on the C' system.