Proteomic Profiling of Plasmodium Sporozoite Maturation Identifies New Proteins Essential for Parasite Development and Infectivity
Proteomic Profiling of Plasmodium Sporozoite Maturation Identifies New Proteins Essential for Parasite Development and Infectivity
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DOI：
10.1371/journal.ppat.1000195
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发表时间：
2008-10-01
期刊：
影响因子：
6.7
通讯作者：
Stunnenberg, Hendrik G.
中科院分区：
文献类型：
--
作者：
Lasonder, Edwin;Janse, Chris J.;Stunnenberg, Hendrik G.
恶性疟原虫子孢子在按蚊体内发育和成熟，引发人类疟疾感染。在这里，我们报告的第一个蛋白质组学比较不同的寄生虫阶段的蚊子早期和晚期卵囊中肠子孢子，成熟的，传染性的唾液腺子孢子。尽管中肠和唾液腺子孢子之间的形态相似，它们的蛋白质组是显着不同的，在协议中增加肝细胞感染性。不同的子孢子蛋白质组含有大量的阶段特异性蛋白质，其注释表明参与子孢子成熟、运动性、人类宿主的感染和相关的代谢调节。通过在啮齿类疟原虫伯氏疟原虫（P.berghei）中的orthoprotein基因破坏，对在恶性疟原虫子孢子蛋白质组中鉴定的蛋白质进行分析，揭示了三种以前未表征的疟原虫蛋白质，它们似乎是蚊子中不同成熟点的子孢子发育所必需的。这项研究揭示了按蚊体内疟原虫的发育和成熟，并确定了可能对子孢子感染人类至关重要的蛋白质。
Plasmodium falciparum sporozoites that develop and mature inside an Anopheles mosquito initiate a malaria infection in humans. Here we report the first proteomic comparison of different parasite stages from the mosquito-early and late oocysts containing midgut sporozoites, and the mature, infectious salivary gland sporozoites. Despite the morphological similarity between midgut and salivary gland sporozoites, their proteomes are markedly different, in agreement with their increase in hepatocyte infectivity. The different sporozoite proteomes contain a large number of stage specific proteins whose annotation suggest an involvement in sporozoite maturation, motility, infection of the human host and associated metabolic adjustments. Analyses of proteins identified in the P. falciparum sporozoite proteomes by orthologous gene disruption in the rodent malaria parasite, P. berghei, revealed three previously uncharacterized Plasmodium proteins that appear to be essential for sporozoite development at distinct points of maturation in the mosquito. This study sheds light on the development and maturation of the malaria parasite in an Anopheles mosquito and also identifies proteins that may be essential for sporozoite infectivity to humans.