Type III Secretion System Translocon Component EseB Forms Filaments on and Mediates Autoaggregation of and Biofilm Formation by Edwardsiella tarda
Type III Secretion System Translocon Component EseB Forms Filaments on and Mediates Autoaggregation of and Biofilm Formation by Edwardsiella tarda
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DOI：
10.1128/aem.01254-15
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发表时间：
2015-09-01
影响因子：
4.4
通讯作者：
Xie, Hai Xia
中科院分区：
文献类型：
--
作者：
Gao, Zhi Peng;Nie, Pin;Xie, Hai Xia
迟缓爱德华氏菌的III型分泌系统（T3 SS）通过将效应蛋白转运到宿主细胞中而在感染中起重要作用。EseB是效应子转位所需的组分，据报道介导E.迟到。在这项研究中，我们证明EseB在E. tarda，是E.在Dulbecco改良的Eagle培养基（DMEM）中培养。E.在DMEM中的tarda不需要FlhB，FlhB是组装鞭毛的必需组分。EseB细丝形成、自聚集和生物膜形成的动态分析表明，EseB细丝的形成发生在自聚集和生物膜形成之前。将EseB抗体添加到E.在细菌自动聚集之前的迟缓培养物以剂量依赖性方式防止自动聚集和生物膜形成，而向E.已经形成生物膜的迟缓培养物不破坏生物膜。因此，EseB介导的细菌细胞-细胞相互作用是自聚集和生物膜形成的先决条件。
The type III secretion system (T3SS) of Edwardsiella tarda plays an important role in infection by translocating effector proteins into host cells. EseB, a component required for effector translocation, is reported to mediate autoaggregation of E. tarda. In this study, we demonstrate that EseB forms filamentous appendages on the surface of E. tarda and is required for biofilm formation by E. tarda in Dulbecco's modified Eagle's medium (DMEM). Biofilm formation by E. tarda in DMEM does not require FlhB, an essential component for assembling flagella. Dynamic analysis of EseB filament formation, autoaggregation, and biofilm formation shows that the formation of EseB filaments occurs prior to autoaggregation and biofilm formation. The addition of an EseB antibody to E. tarda cultures before bacterial autoaggregation prevents autoaggregation and biofilm formation in a dose-dependent manner, whereas the addition of the EseB antibody to E. tarda cultures in which biofilm is already formed does not destroy the biofilm. Therefore, EseB filament-mediated bacterial cell-cell interaction is a prerequisite for autoaggregation and biofilm formation.