Overexpressing Exogenous 5-Enolpyruvylshikimate-3-Phosphate Synthase (EPSPS) Genes Increases Fecundity and Auxin Content of Transgenic Arabidopsis Plants.
Overexpressing Exogenous 5-Enolpyruvylshikimate-3-Phosphate Synthase (EPSPS) Genes Increases Fecundity and Auxin Content of Transgenic Arabidopsis Plants.
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过表达外源 5-烯醇丙酮莽草酸-3-磷酸合酶 (EPSPS) 基因可提高转基因拟南芥植物的繁殖力和生长素含量
DOI：
10.3389/fpls.2018.00233
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发表时间：
2018
影响因子：
5.6
通讯作者：
Lu BR
中科院分区：
文献类型：
--
作者：
Fang J;Nan P;Gu Z;Ge X;Feng YQ;Lu BR
过量产生EPSPS(5-烯醇丙酮基莽草酸-3-磷酸合成酶)的转基因耐草甘膦植物可能会在无草甘膦环境中表现出更强的适应性。如果是这样的话，将过量表达EPSPS的转基因导入到野生近缘物种中可能会导致作物-野生杂交种竞争力的增强，导致不可预测的环境影响。评估过量表达EPSPS的转基因在模式植物物种中的适合性效应有助于解决这个问题，同时阐明过量表达EPSPS如何影响植物的适合性相关性状。我们获得了从水稻或农杆菌(CP4)中分离到的T2和T3系，分别带有或不带有过表达EPSPS的转基因。对于这三个转基因中的每一个，我们在普通花园实验中比较了转基因存在、没有转基因、空载体(EV)和亲本系中的草甘膦耐受性、一些与适应性相关的性状和生长素(吲哚-3-乙酸)含量。我们检测到过量产生EPSPS的转基因家系T2植株对草甘膦的耐受性显著提高。我们还记录了水稻繁殖力的显著增加，这与含有水稻EPSPS基因的T3转基因家系中生长素含量的增加有关，与其分离的转基因缺失家系、EV和亲本对照相比。我们对9个转基因事件的拟南芥的研究结果有力地支持了这样的假设，即在无草甘膦环境中，过量生产EPSPS的转基因植物可以受益于繁殖力优势。过量生产EPSPS刺激植物生长素的生物合成，可能在提高转基因拟南芥植株的繁殖力方面发挥作用。所获得的知识有助于评估从任何转基因作物向其野生近缘种群中导入过量产生EPSP的转基因所造成的环境影响。
Transgenic glyphosate-tolerant plants overproducing EPSPS (5-enolpyruvylshikimate-3-phosphate synthase) may exhibit enhanced fitness in glyphosate-free environments. If so, introgression of transgenes overexpressing EPSPS into wild relative species may lead to increased competitiveness of crop-wild hybrids, resulting in unpredicted environmental impact. Assessing fitness effects of transgenes overexpressing EPSPS in a model plant species can help address this question, while elucidating how overproducing EPSPS affects the fitness-related traits of plants. We produced segregating T2 and T3 Arabidopsis thaliana lineages with or without a transgene overexpressing EPSPS isolated from rice or Agrobacterium (CP4). For each of the three transgenes, we compared glyphosate tolerance, some fitness-related traits, and auxin (indole-3-acetic acid) content in transgene-present, transgene-absent, empty vector (EV), and parental lineages in a common-garden experiment. We detected substantially increased glyphosate tolerance in T2 plants of transgene-present lineages that overproduced EPSPS. We also documented significant increases in fecundity, which was associated with increased auxin content in T3 transgene-present lineages containing rice EPSPS genes, compared with their segregating transgene-absent lineages, EV, and parental controls. Our results from Arabidopsis with nine transgenic events provide a strong support to the hypothesis that transgenic plants overproducing EPSPS can benefit from a fecundity advantage in glyphosate-free environments. Stimulated biosynthesis of auxin, an important plant growth hormone, by overproducing EPSPS may play a role in enhanced fecundity of the transgenic Arabidopsis plants. The obtained knowledge is useful for assessing environmental impact caused by introgression of transgenes overproducing EPSPS from any GE crop into populations of its wild relatives.
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