The mutant of sll1961, which encodes a putative transcriptional regulator, has a defect in regulation of photosystem stoichiometry in the cyanobacterium Synechocystis sp PCC 6803
The mutant of sll1961, which encodes a putative transcriptional regulator, has a defect in regulation of photosystem stoichiometry in the cyanobacterium Synechocystis sp PCC 6803
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DOI：
10.1104/pp.105.064782
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发表时间：
2005-09-01
期刊：
影响因子：
7.4
通讯作者：
Sonoike, K
中科院分区：
文献类型：
--
作者：
Fujimori, T;Higuchi, M;Sonoike, K
在适应变化的光环境中，光合生物调节两个光合反应中心（光系统IIPSI和光系统II）的比例。从随机转座子诱变产生的突变体中分离出一种突变体，该突变体在向强光转移时不能调节光系统化学计量。通过对突变体叶绿素荧光的测定和对PSI反应中心亚基的western blotting分析，发现该突变体在强光条件下PSI含量不受抑制。在突变体中，转座子被插入到编码推定转录调节因子的sll1961基因中。基因芯片分析表明，sll 1961突变体在强光照射3 h后，sll 1773的表达被显著诱导。我们的研究结果表明，转录调节，Sll 1961，其可能的靶蛋白，包括Sll 1773，可能是负责调节光系统的化学计量响应于强光。
In acclimation to changing light environments, photosynthetic organisms modulate the ratio of two photosynthetic reaction centers ( photosystem IIPSI] and photosystem II). One mutant, which could not modulate photosystem stoichiometry upon the shift to high light, was isolated from mutants created by random transposon mutagenesis. Measurements of chlorophyll fluorescence and analysis of the reaction center subunits of PSI through western blotting in this mutant revealed that the content of PSI could not be suppressed under high-light condition. In the mutant, transposon was inserted to the sll1961 gene encoding a putative transcriptional regulator. DNA microarray analysis revealed that the expression of sll1773 was drastically induced in the sll1961 mutant upon exposure to high light for 3 h. Our results demonstrate that a transcriptional regulator, Sll1961, and its possible target proteins, including Sll1773, may be responsible for the regulation of photosystem stoichiometry in response to high light.