Detection of aberrations of the p53 alleles and the gene transcript in human tumor cell lines by single-strand conformation polymorphism analysis.
Detection of aberrations of the p53 alleles and the gene transcript in human tumor cell lines by single-strand conformation polymorphism analysis.
复制标题
通过单链构象多态性分析检测人肿瘤细胞系中 p53 等位基因和基因转录物的畸变。
作者：
Yoshinori S. Murakami;Kenshi Hayashi;T. Sekiya
采用聚合酶链式反应产物单链构象多态性分析方法，检测了10株人肿瘤细胞系中p53基因及其转录本的突变情况。这种方法可以同时检测到两个等位基因中的一个丢失和剩余等位基因中的一个点突变。在4个肿瘤细胞系中发现了新的异常。三个细胞系失去了其中一个等位基因，另一个发生了点突变。这些导致氨基酸替换的点突变位于P53基因区域，该区域在不同物种中高度保守。所有突变基因均获得表达，并产生点突变的mRNAs。一个细胞系没有检测到任何可检测到的p53基因转录本，尽管在分析的区域没有检测到该基因的结构异常。在这项工作中被发现携带p53基因异常的大多数细胞系之前都被报道在癌基因或除p53基因之外的肿瘤抑制基因中存在突变。这些结果提供了人类肿瘤发生过程中多种基因变化的更多例子。
Aberrations of the p53 gene and its transcript in ten human tumor-derived cell lines were investigated by single-strand conformation polymorphism analysis of polymerase chain reaction products. This method can detect loss of one of the two alleles and a point mutation in the remaining allele simultaneously. Aberrations were newly found in four tumor cell lines. Three cell lines had lost one of the alleles and had a point mutation in the other. These point mutations, resulting in amino acid substitutions, were in the region of the p53 gene, which is highly conserved in different species. All the mutated genes were expressed and produced mRNAs with point mutations. One cell line did not contain any detectable transcript of the p53 gene, although no structural aberration of the gene was detected in the regions analyzed. Most of the cell lines found to carry aberrations of the p53 gene in this work have previously been reported to have mutations in oncogenes or in a tumor suppressor gene other than the p53 gene. These results provide additional examples of multiple genetic changes in the genesis of human tumors.
影响因子：
56.9
作者：
MALKIN, D;LI, FP;FRIEND, SH
通讯作者：
FRIEND, SH
DOI：
10.1073/pnas.87.5.1973
发表时间：
1990-03-01
影响因子：
11.1
作者：
BRESSAC, B;GALVIN, KM;OZTURK, M
通讯作者：
OZTURK, M