THE AUTOMATIC THIN SECTIONING AND STAINING SYSTEM FOR LIGHT MICROSCOPY : THE TESTS BY TWO TRIAL PRODUCTS
THE AUTOMATIC THIN SECTIONING AND STAINING SYSTEM FOR LIGHT MICROSCOPY : THE TESTS BY TWO TRIAL PRODUCTS
复制标题
光学显微镜自动薄切片染色系统：两种试用产品的测试
DOI：
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
H. Nanto
中科院分区：
文献类型：
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作者：
M. Kokubo;T. Higuchi;K. Kudoh;Yoshimitsu Fukuda;H. Nanto
在病理学中，组织切片的制备是绝对必要的。然而，这个过程仍然主要是手动执行的。从大量标本中制备许多样品需要细致的技能。另外，很难保持产品的恒定精度。为了解决这些问题，我们提出了两种新的自动高速制样试验机。第一种设备在概念上是基于在切片之前将压敏胶带附着在石蜡块的表面上。整个过程可以自动重复。切片由新开发的高精度自动切片机进行。使用该设备，切片可以连续地贴在胶带上并自动染色。我们还开发了第二台机器，利用静电自动将切片贴在目标玻璃上。该方法的原理是基于对石蜡块的表面进行静电充电，通过该静电充电，绝缘带被吸引并附着到石蜡块的表面。在对块体进行分段之后，该分段通过静电力保持粘附在绝缘带上。随后，该部分也通过使用静电转移到目标玻璃上。在使用这种方法时，可以使常规物镜上的组织切片完全自动化。
The preparation of tissue sections for light microscopy is absolutely necessary in pathology. However, this process is still mainly performed manually. The preparation of many samples from a large number of specimens requires meticulous skills. In addition, it is very difficult to maintain a constant accuracy of the producedure.To solve these problems we propose two new test machineries for automatic highspeed sample preparations. The first apparatus is conceptually based on attaching pressure-sensitive adhesive tape on the surface of a paraffin block prior to sectioning. This whole process could be repeated automatically. The sectioning is performed by a newly developed high-precision automatic microtome. Using the proposed machine, cut sections could be continuously mounted on the tape and stained automatically.We also developed the second machine to mount sections on the object glass automatically by using static electricity. The principle of this method is based on electrostatically charging the surface of a paraffin block by which insulation tape was attracted and attached to the surface of the block. After sectioning the block, the section remains adhered on the insulation tape by electrostatic forces. Subsequently, the section is transferred to the object glass by using static electricity as well. In the use of such methods, tissue section on conventional object glass could be made fully automatic.