A Cross-Sectional Study to Correlate Disease Severity in Bullous Pemphigoid Patients with Serum Levels of Autoantibodies Against BP180 and BP230.
A Cross-Sectional Study to Correlate Disease Severity in Bullous Pemphigoid Patients with Serum Levels of Autoantibodies Against BP180 and BP230.
复制标题
大疱性类天疱疮患者疾病严重程度与血清抗BP180和BP230自身抗体水平相关性的横断面研究
DOI：
10.4103/idoj.idoj_813_20
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发表时间：
2021-09
影响因子：
1.7
通讯作者：
Khopkar US
中科院分区：
文献类型：
--
作者：
Muhammed N;Korgaonkar S;Pradhan V;Khopkar US
大疱性类天疱疮患者通常采用酶联免疫吸附试验(ELISA)检测BP180和230抗体。我们找不到太多关于这种测试在印度人口中预测疾病严重程度的有用性的数据。目的：探讨大疱性类天疱疮患者血清中抗BP180、BP30抗体滴度与病情严重程度及临床特征的关系。这项横断面研究是在印度西部的一家三级护理中心进行的。对42例临床诊断为大疱性类天疱疮的初治病例进行了皮肤穿刺法、Ig G抗BP180、抗BP230双抗体夹心法、直接免疫荧光法和间接免疫荧光法的检测。通过计算改良的自身免疫性大疱性皮肤疾病强度评分(Absis)来评估疾病的严重程度。30名最终诊断为大疱性类天疱疮的患者纳入统计分析。采用皮尔逊相关系数(R)进行相关性研究。两项试验均为阴性时的平均皮肤评分为32.81分，仅BP30阳性时为42.13分，BP180为阳性时为76.28分，两者均为阳性时为78.16分。BP180与Absis皮肤评分的相关系数r=0.60(P值：0.0005)，BP230与皮肤评分的相关系数r=-0.055(P值：0.600)。BP抗体滴度与疾病严重程度部分相关。抗BP180抗体与更严重的疾病有关。抗BP230抗体滴度与疾病严重程度无关。
Enzyme-linked immunosorbent assay (ELISA) for BP 180 and 230 antibodies is commonly done in patients with bullous pemphigoid. We could not find much data regarding the usefulness of this test to predict the disease severity in Indian population. We studied the correlation of IgG anti BP180 and anti BP230 antibody titer with disease severity and clinical features in bullous pemphigoid. This cross-sectional study was conducted at a tertiary care center in western India. Forty-two clinically diagnosed treatment-naive cases of bullous pemphigoid were enrolled and investigated with skin punch biopsy, IgG anti BP180, and anti BP230 ELISA, direct immunofluorescence, and indirect immunofluorescence tests. Disease severity was assessed by calculating modified Autoimmune Bullous Skin Disorder Intensity Score (ABSIS) score. Thirty patients with a final diagnosis of bullous pemphigoid were included in the statistical analysis. Pearson's correlation coefficient (r) was used to study correlation. The mean ABSIS skin score was 32.81 when both tests were negative, 42.13 when only BP230 was positive, 76.28 when only BP180 was positive, and 78.16 when both were positive. Pearson's correlation coefficient (r) for BP180 and ABSIS skin score was 0.6 (P value: 0.0005), and for BP230 was -0.055 (P value: 0.600). BP antibody titers correlate partially with disease severity. Anti-BP180 antibody is associated with more severe disease. Anti-BP230 antibody titer does not correlate with disease severity.