G6PD activity and gene expression in leukemic cells from G6PD-deficient subjects
G6PD activity and gene expression in leukemic cells from G6PD-deficient subjects
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DOI：
10.1016/s0304-3835(99)00052-x
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发表时间：
1999-06-01
期刊：
影响因子：
9.7
通讯作者：
Dessì, S
中科院分区：
文献类型：
--
作者：
Batetta, B;Pulisci, D;Dessì, S
在本研究中，我们检测了从G6PD正常和缺乏的受试者中分离的白血病细胞的基因表达和葡萄糖-6-磷酸脱氢酶（G6PD）活性。结果表明，与外周血单个核细胞（PBMC）相比，G6PD在G6PD正常白血病细胞和G6PD缺陷白血病细胞中的活性明显增加。与G6PD正常患者相比，G6PD缺陷患者白血病细胞中G6PD基因表达水平较高。3-羟基-3-甲基戊二酰辅酶A （HMGCoA）还原酶也观察到类似的基因表达模式。这些结果支持了G6PD缺陷细胞为了维持其生长，必须通过诱导基因表达来响应其突变酶的低活性，增加其数量。1999爱思唯尔科学爱尔兰有限公司版权所有。
In the present study we examined gene expression and glucose-6-phosphate dehydrogenase (G6PD) activity in leukemic cells isolated from G6PD normal and deficient subjects. The results have shown that G6PD activity strongly increases in G6PD normal leukemic cells as well as in G6PD deficient leukemic cells when compared to peripheral blood mononuclear cells (PBMC). Higher levels of G6PD gene expression were observed in leukemic cells from G6PD deficient patients compared to G6PD normal. A similar pattern of gene expression was also observed for 3-hydroxy-3-methylglutaryl coenzyme A (HMGCoA) reductase. These results support the hypothesis that G6PD deficient cell, in order to sustain their growth, must respond to the low activity of their mutant enzyme with an increase in quantity through an induction of gene expression. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.