One-pot fluorescent assay for sensitive detection of APOBEC3A activity.
One-pot fluorescent assay for sensitive detection of APOBEC3A activity.
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用于灵敏检测 APOBEC3A 活性的一锅荧光测定法
DOI:
10.1039/d1cb00076d
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发表时间:
2021-08-05
影响因子:
4.1
通讯作者:
Zhou X
中科院分区:
文献类型:
--
作者:
Wang B;Zhang X;Wang Y;Chen K;Wang F;Weng X;Zhou X
We reported a one-pot fluorescence-based assay to quantitively detect A3A activity combined with cytosine deamination and uracil excision. After deamination by A3A and USER enzyme treatment, the fluorescent turn-on effect at 520 nm was observed, which can be used to evaluate the A3A activity and screen inhibitors.
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