Quantitative time-course proteome analysis of Mesorhizibium loti during nodule maturation
Quantitative time-course proteome analysis of Mesorhizibium loti during nodule maturation
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Mesorhizibium loti 根瘤成熟过程中的定量时程蛋白质组分析
DOI：
10.1016/j.jprot.2015.04.034
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发表时间：
2015
影响因子：
3.3
通讯作者：
Mitsuyoshi Ueda
中科院分区：
文献类型：
--
作者：
Mami Nambu;Yohei Tatsukami;Hironobu Morisaka;Kouichi Kuroda;Mitsuyoshi Ueda
根瘤菌是与豆科植物建立共生关系的固氮细菌。为了解根瘤菌改变自身代谢从而与寄主建立成功的固氮共生关系的机制，以百脉根为材料，接种中生根瘤菌（Mesorhizobiumloti）。从接种后2周（结节发育的早期阶段）、3周和4周（结节发育的中期阶段）收获的结节中分离类杆菌。使用定量时程蛋白质组学分析，我们定量了537个蛋白质在M中产生的变化。根瘤成熟过程中的类梭杆菌。结果表明，在不同的温度下，M. lotiupon分化为拟杆菌。此外，我们的研究结果表明，M.在结核发育的早期阶段，lotin进入缺氮状态，然后在结核发育的中期阶段进入富氮状态。此外，我们的数据表明，M.在结核发育的中间阶段，我们的研究结果提供了新的见解的过程中所经历的生理转变由M。在根瘤成熟期间，
Rhizobia are nitrogen-fixing bacteria that establish a symbiotic relationship with leguminous plants. To understand the mechanism by which rhizobia alter their metabolism to establish successful nitrogen-fixing symbiotic relationship with hosts,Lotus japonicuswere inoculated withMesorhizobiumloti. Bacteroids were isolated from nodules harvested at 2 weeks (the early stage of nodule development), and at 3 and 4 weeks (the intermediate stage of nodule development) post-inoculation. Using a quantitative time-course proteome analysis, we quantified the variations in the production of 537 proteins inM. lotibacteroids during the course of nodule maturation. The results revealed significant changes in the carbon and amino acid metabolisms byM. lotiupon differentiating into bacteroids. Furthermore, our findings suggested thatM. lotienters a nitrogen-deficient condition during the early stages of nodule development, and then a nitrogen-rich condition during the intermediate stages of nodule development. In addition, our data indicated thatM. lotiassimilated ammonia during the intermediate stages of nodule development. Our results provide new insights into the course of physiological transitions undergone byM. lotiduring nodule maturation.