Expression of an alpha-1,3-glucanase during mycoparasitic interaction of Trichoderma asperellum.
Expression of an alpha-1,3-glucanase during mycoparasitic interaction of Trichoderma asperellum.
复制标题
木霉寄生相互作用过程中 α-1,3-葡聚糖酶的表达。
DOI：
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
E. Monte
中科院分区：
文献类型：
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作者：
L. Sanz;M. Montero;J. Redondo;A. Llobell;E. Monte
木霉属物种由于具有拮抗植物病原真菌的能力，已被研究作为生物防治剂70多年。真菌寄生是木霉菌株的拮抗活性的主要机制之一，它依赖于能够降解宿主细胞壁的水解酶的复杂混合物的分泌。α-1，3-葡聚糖酶（EC 3.2.1.59，能够降解α-1，3-葡聚糖的酶，也称为突变酶）的抗真菌活性已在T.哈茨氏菌及其在真菌寄生过程中的作用。本文报道了拮抗真菌T. Asperellum T32.在针对草莓病原体灰葡萄孢的对抗试验中，在转录水平上的表达强烈支持AGN13.2在T.棘孢菌属
Trichoderma species have been investigated as biological control agents for over 70 years owing to their ability to antagonize plant pathogenic fungi. Mycoparasitism, one of the main mechanisms involved in the antagonistic activity of Trichoderma strains, depends on the secretion of complex mixtures of hydrolytic enzymes able to degrade the host cell wall. The antifungal activity of an alpha-1,3-glucanase (EC 3.2.1.59, enzymes able to degrade alpha-1,3-glucans and also named mutanases) has been described in T. harzianum and its role in mycoparasitic processes has been suggested. In this study, we report on the purification, characterization and cloning of an exo-alpha-1,3-glucanase, namely AGN13.2, from the antagonistic fungus T. asperellum T32. Expression at the transcription level in confrontation assays against the strawberry pathogen Botrytis cinerea strongly supports the role of AGN13.2 during the antagonistic action of T. asperellum.