Antigenic and immunogenic properties of truncated VP28 protein of white spot syndrome virus in Procambarus clarkii
Antigenic and immunogenic properties of truncated VP28 protein of white spot syndrome virus in Procambarus clarkii
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克氏原螯虾白斑综合征病毒VP28截短蛋白的抗原性和免疫原性
DOI：
10.1016/j.fsi.2012.11.007
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发表时间：
2013-01-01
影响因子：
4.7
通讯作者：
Wang, Yi-Zhen
中科院分区：
文献类型：
--
作者：
Du, Hua-Hua;Hou, Chong-Lin;Wang, Yi-Zhen
以往的研究发现，对虾白斑综合征病毒(WSSV)的VP28囊膜蛋白是其主要抗原蛋白。尽管与病毒感染性有关，但其功能作用尚不清楚。在本研究中，我们描述了重组截短的VP28蛋白的多克隆抗体的制备，包括缺失的N端(RVP28 Delta N)、C端(RVP28 Delta C)和中间(RVP28 Delta M)。在抗原性分析中，缺乏N-末端或中间区域的VP28截短产生的抗体显著降低了克氏原虾对WSSV的中和力。进一步的免疫原性分析表明，在攻击WSSV之前，接种这些截短片段的小龙虾的相对存活率(RPS)降低。这些结果表明，N末端(残基1-27)和中间区域(残基35-95)是维持VP28中和性线性表位所必需的，并参与免疫应答。因此，这些区域最有可能暴露在VP28上，这将有助于合理设计针对WSSV VP28的有效疫苗。(C)2012爱思唯尔有限公司。保留所有权利。
Previous studies identify VP28 envelope protein of white spot syndrome virus (WSSV) as its main antigenic protein. Although implicated in viral infectivity, its functional role remains unclear. In the current study, we described the production of polyclonal antibodies to recombinant truncated VP28 proteins including deleted N-terminal (rVP28 Delta N), C-terminal (rVP28 Delta C) and middle (rVP28 Delta M). In antigenicity assays, antibodies developed from VP28 truncations lacking the N-terminal or middle regions showed significantly lowered neutralization of WSSV in crayfish, Procambarus clarkii. Further immunogenicity analysis showed reduced relative percent survival (RPS) in crayfish vaccinating with these truncations before challenge with WSSV. These results indicated that N-terminal (residues 1-27) and middle region (residues 35-95) were essential to maintain the neutralizing linear epitopes of VP28 and responsible in eliciting immune response. Thus, it is most likely that these regions are exposed on VP28, and will be useful for rational design of effective vaccines targeting VP28 of WSSV. (c) 2012 Elsevier Ltd. All rights reserved.