A CDNA CLONE EXPRESSING A RHOPTRY PROTEIN OF PLASMODIUM-FALCIPARUM
A CDNA CLONE EXPRESSING A RHOPTRY PROTEIN OF PLASMODIUM-FALCIPARUM
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DOI：
10.1016/0166-6851(87)90020-x
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发表时间：
1987-08-01
影响因子：
1.5
通讯作者：
KEMP, DJ
中科院分区：
文献类型：
--
作者：
COPPEL, RL;BIANCO, AE;KEMP, DJ
利用免疫人的抗体选择表达恶性疟原虫无性血期抗原的cDNA克隆。用表达的融合多肽作为亲和试剂纯化相应寄生虫抗原特异性的人抗体。Western blotting和免疫电镜结果表明，该抗原是一个位于裂殖子菱形体中的105kda蛋白。cDNA编码弓形虫抗原的羧基端，该序列富含带电氨基酸和羟基氨基酸。
Antibodies from immune humans were used to select a cDNA clone expressing an asexual blood stage antigen of Plasmodium falciparum. The expressed fused polypeptide was used as an affinity reagent to purify human antibodies specific for the corresponding parasite antigen. Western blotting and immunoelectronmicroscopy demonstrated that the antigen was a 105 kDa protein located in the rhoptries of merozoites. The cDNA encodes the carboxy terminus of the rhoptry antigen, a sequence rich both in charged and hydroxy amino acids.