Babesia microti:: Prevalence in wild rodents and Ixodes ricinus ticks from the Mazury Lakes District of north-eastern Poland
Babesia microti:: Prevalence in wild rodents and Ixodes ricinus ticks from the Mazury Lakes District of north-eastern Poland
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DOI：
10.1016/j.ijmm.2006.01.015
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发表时间：
2006-05-01
影响因子：
4.1
通讯作者：
Behnke, Jerzy M.
中科院分区：
文献类型：
--
作者：
Sinski, Edward;Bajer, Anna;Behnke, Jerzy M.
在波兰，一种常见的泛北极啮齿动物红寄生原虫--在北美、欧洲和欧亚大陆的北方温带地区，有充分的文献证明，这种寄生虫存在于各种野生啮齿动物中。然而，人类巴贝斯虫病可归因于B的感染。据报道，Microti仅产于美国东北部和中西部偏上地区以及日本。我们最近进行了一项流行病学调查，调查B的流行情况。在波兰东北部的蜱类和野生啮齿动物中发现了microti。从总共483只动物中采集血样，包括三个物种：黄颈姬鼠、田鼠和被困在马祖里湖区Mikolajki附近的Urwitalt的田鼠。奎斯特成人岛在研究地点，通过在异质落叶林地的植被上进行地毯式拖曳收集蓖麻蜱，此外，还仔细检查啮齿动物的幼虫和幼虫摄食情况。在1513株I. PCR检测蓖麻蜱（0.6%）。其中包括163只成虫（92只雌性和71只雄性）、50只成虫和1300只幼虫，PCR阳性率分别为3%、8%和0%。85 A. flavicollis，374 M. arvalis和24 M. 1%，12.8%和42%的oeconomus是寄生虫，分别通过显微镜检查的血涂片染色与姬姆萨。B。从53株M. arvalis和5 M. oeconomus和巢式PCR检测，靶向piroplasm特异性部分的18 S核糖体DNA，分别显示72%和40%，是PCR阳性。序列分析表明，所有PCR阳性样品的rDNA序列与慕尼黑B相同（100%同源）。分离自小家鼠的田鼠菌株（基因库登录号AB 071177）。本研究结果表明，B.田鼠属中常见的田鼠。啮齿类动物可能不是人畜共患菌株，因此，它不太可能对波兰东北部Mazury湖区的公共卫生构成风险。(c)2006年Elsevier GmbH。All rights reserved.
Infections of Babesia microti (Apicomplexa, Piroplasmida), a common erythroparasitic protozoan of Holarctic rodents, are not widely acknowledged in Poland. The presence of this parasite in various species of wild rodents has been well documented throughout the northern temperate zone of North America, Europe, and Eurasia. However, human babesiosis attributable to infection with B. microti has been reported only from the north-eastern and upper midwestern United States and Japan. We recently carried out an epizootiological survey investigating the prevalence of B. microti both in the tick Ixodes ricinus and in wild rodents in north-eastern Poland. Blood samples were collected from a total of 483 animals comprising three species: Apodemus flavicollis, Microtus arvalis, and Microtus oeconomus trapped at Urwitalt near Mikolajki in the Mazury Lakes District. Questing adult I. ricinus ticks were collected in the study sites by blanket dragging of vegetation in heterogeneous, deciduous woodland, and, in addition, rodents were carefully examined for feeding larvae and nymphs. Altogether, R microti was detected in 9 out of 1513 I. ricinus ticks (0.6%) examined by PCR. This included 163 adults (92 females and 71 males), 50 nymphs, and 1300 larvae 3%, 8%, and 0% of which were PCR-positive, respectively. Of 85 A. flavicollis, 374 M. arvalis and 24 M. oeconomus, 1%, 12.8%, and 42% were parasitaemic, respectively, as determined by microscopic examination of blood smears stained with Giemsa. B. microti DNA, extracted from 53 M. arvalis and 5 M. oeconomus and examined by nested PCR, targeting a piroplasm-specific portion of the 18S ribosomal DNA, revealed 72% and 40%, respectively, to be PCR positive. Sequence analysis showed that all PCR-positive samples had rDNA sequences identical (100% homology) to that of the Munich B. microti strain (GenBank Accession no. AB071177) isolated from Mus musculus. The results of this study indicate that the B. microti commonly encountered among Microtus spp. rodents is probably not a zoonotic strain and, therefore, that it is most unlikely to represent a risk to public health in the Mazury Lakes District of northeastern Poland. (c) 2006 Elsevier GmbH. All rights reserved.