Different regulation of T-box genes Tbx4 and Tbx5 during limb development and limb regeneration
Different regulation of T-box genes Tbx4 and Tbx5 during limb development and limb regeneration
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DOI：
10.1006/dbio.2002.0801
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发表时间：
2002-10-15
影响因子：
2.7
通讯作者：
Simon, HG
中科院分区：
文献类型：
--
作者：
Khan, P;Linkhart, B;Simon, HG
T结构域转录因子Tbx4和Tbx5由于其肢体型特异性表达而分别参与控制脊椎动物腿和臂的身份。为了研究这些基因在肢体发育和再生中的作用，我们从蝾螈中克隆了Tbx4和Tbx5 cDNA，并产生了识别Tbx4或Tbx5蛋白的抗血清。我们在这里表明，在两个有尾目两栖动物，蝾螈和蝾螈，Tbx4和Tbx5的调节不同于高等脊椎动物。在mRNA和蛋白质水平上，Tbx4和Tbx5都在发育中的后肢和前肢中表达。这些基因的共表达表明，额外的因素参与了肢体类型特异性模式的控制。此外，蝾螈和蝾螈Tbx4和Tbx5的表达在胚胎发生和再生形态发生过程中受到不同的调节。在再生过程中，Tbx5仅在前肢中上调，而Tbx4仅在后肢中上调。这表明，在分子水平上，不同的调控机制控制着相同肢体结构的形成，再生不仅仅是发育基因程序的重复。(C)2002 Elsevier Science（美国）。
The T-domain transcription factors Tbx4 and Tbx5 have been implicated, by virtue of their limb-type specific expression, in controlling the identity of vertebrate legs and arms, respectively. To study the roles of these genes in developing and regenerating limbs, we cloned Tbx4 and Tbx5 cDNAs from the newt, and generated antisera that recognize Tbx4 or Tbx5 proteins. We show here that, in two urodele amphibians, newts and axolotls, the regulation of Tbx4 and Tbx5 differs from higher vertebrates. At the mRNA and protein level, both Tbx4 and Tbx5 are expressed in developing hindlimbs as well as in developing forelimbs. The coexpression of these genes argues that additional factors are involved in the control of limb type-specific patterns. In addition, newt and axolotl Tbx4 and Tbx5 expression is regulated differently during embryogenesis and regenerative morphogenesis. During regeneration, Tbx5 is exclusively upregulated in the forelimbs, whereas Tbx4 is exclusively upregulated in the hindlimbs. This indicates that, on a molecular level, different regulatory mechanisms control the shaping of identical limb structures and that regeneration is not simply a reiteration of developmental gene programs. (C) 2002 Elsevier Science (USA).