Monohydroxylated polycyclic aromatic hydrocarbons inhibit both osteoclastic and osteoblastic activities in teleost scales.
Monohydroxylated polycyclic aromatic hydrocarbons inhibit both osteoclastic and osteoblastic activities in teleost scales.
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DOI：
10.1016/j.lfs.2009.01.008
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发表时间：
2009-03
期刊：
影响因子：
6.1
通讯作者：
N. Suzuki;K. Hayakawa;T. Kameda;Akira Triba;N. Tang;M. Tabata;K. Takada;S. Wada;Katsunori Omori;A. Srivastav;H. Mishima;A. Hattori
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文献类型：
--
作者：
N. Suzuki;K. Hayakawa;T. Kameda;Akira Triba;N. Tang;M. Tabata;K. Takada;S. Wada;Katsunori Omori;A. Srivastav;H. Mishima;A. Hattori
目的利用酵母双杂交技术研究了单羟基多环芳烃（OHPAH）与雌激素受体（ER）的结合活性，但多环芳烃不具有结合活性。因此，检查了3-羟基苯并[a]蒽（3-OHBaA）和4-羟基苯并[a]蒽（4-OHBaA）对硬骨鱼中破骨细胞和成骨细胞的直接作用。作为阴性对照，1-羟基芘（1-OHPy），它没有结合活性的人ER，used.MAIN METHODS的OHPHs对破骨细胞和成骨细胞的影响进行了检查，通过使用硬骨鱼规模作为每个标志物的测定系统：抗酒石酸酸性磷酸酶的破骨细胞和成骨细胞的碱性磷酸酶。组织蛋白酶K的变化（一种骨细胞标志物）和胰岛素样生长因子-I（IGF-I）通过逆转录-聚合酶链反应检测了4-OHBaA处理的金鱼鳞片中成骨细胞标志物mRNA的表达。（淡水硬骨鱼）和濑鱼（一种海洋硬骨鱼），鳞片中的骨细胞活性被3-OHBaA和4-OHBaA显著抑制，尽管1-OHPy不影响骨细胞活性。关于成骨细胞，3-OHBaA和4-OHBaA均降低成骨细胞活性，而1-OHPy处理未改变成骨细胞活性。然而，17β-雌二醇（E2）显着增加了金鱼和濑鱼鳞片的成骨细胞和成骨细胞的活性。组织蛋白酶K和IGF-I的mRNA表达在4-OHBaA处理的鳞片中降低，但在E2处理的鳞片中升高。
AIMSWe previously demonstrated that monohydroxylated polycyclic aromatic hydrocarbons (OHPAHs) bound to a human estrogen receptor (ER) by a yeast two-hybrid assay, but polycyclic aromatic hydrocarbons did not have a binding activity. Therefore, the direct effect of 3-hydroxybenz[a]anthracene (3-OHBaA) and 4-hydroxybenz[a]anthracene (4-OHBaA) on osteoclasts and osteoblasts in teleosts was examined. As a negative control, 1-hydroxypyrene (1-OHPy), which has no binding activity to human ER, was used.MAIN METHODSThe effect of OHPAHs on osteoclasts and osteoblasts was examined by an assay system using teleost scale as each marker: tartrate-resistant acid phosphatase for osteoclasts and alkaline phosphatase for osteoblasts. Changes in cathepsin K (an osteoclastic marker) and insulin-like growth factor-I (IGF-I) (an osteoblastic marker) mRNA expressions in 4-OHBaA-treated goldfish scales were examined by using a reverse transcription-polymerase chain reaction.KEY FINDINGSIn both goldfish (a freshwater teleost) and wrasse (a marine teleost), the osteoclastic activity in the scales was significantly suppressed by 3-OHBaA and 4-OHBaA, although 1-OHPy did not affect the osteoclastic activity. In reference to osteoblasts, the osteoblastic activity decreased with both 3-OHBaA and 4-OHBaA and did not change with the 1-OHPy treatment. However, 17β-estradiol (E2) significantly increased both the osteoclastic and osteoblastic activities in the scales of both goldfish and wrasse. The mRNA expressions of both cathepsin K and IGF-I decreased in the 4-OHBaA-treated scales but increased in the E2-treated scales.SIGNIFICANCEThe current data are the first to demonstrate that 3-OHBaA and 4-OHBaA inhibited both osteoclasts and osteoblasts and disrupted the bone metabolism in teleosts.