Genotyping-by-sequencing (GBS), an ultimate marker-assisted selection (MAS) tool to accelerate plant breeding.
Genotyping-by-sequencing (GBS), an ultimate marker-assisted selection (MAS) tool to accelerate plant breeding.
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DOI：
10.3389/fpls.2014.00484
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发表时间：
2014
影响因子：
5.6
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
He J;Zhao X;Laroche A;Lu ZX;Liu H;Li Z
分子标记辅助选择（Marker-assisted selection，MAS）是指利用分子标记辅助表型选择进行作物改良。单核苷酸多态性（single nucleotide polymorphism，SNP）等分子标记已被鉴定并有效地应用于植物育种。下一代测序（NGS）技术的应用导致了全基因组测序的显著进步，其提供了超通量序列以彻底改变植物基因分型和育种。为了进一步将NGS的用途扩展到大的作物基因组，例如玉米和小麦，已经开发了测序基因分型（GBS）并将其应用于对多重样品进行测序，所述多重样品结合了联合收割机分子标记发现和基因分型。GBS是NGS方案在作物基因组和群体中发现和基因分型SNP的新应用。GBS方法包括用限制性内切酶消化基因组DNA，然后连接条形码衔接子，PCR扩增和在单泳道流动池上对扩增的DNA池进行测序。需要生物信息学管道来分析和解释GBS数据集。GBS作为一种高性价比的MAS工具，已成功应用于大规模植物育种中的全基因组关联研究、基因组多样性研究、遗传连锁分析、分子标记发现和基因组选择等。
Marker-assisted selection (MAS) refers to the use of molecular markers to assist phenotypic selections in crop improvement. Several types of molecular markers, such as single nucleotide polymorphism (SNP), have been identified and effectively used in plant breeding. The application of next-generation sequencing (NGS) technologies has led to remarkable advances in whole genome sequencing, which provides ultra-throughput sequences to revolutionize plant genotyping and breeding. To further broaden NGS usages to large crop genomes such as maize and wheat, genotyping-by-sequencing (GBS) has been developed and applied in sequencing multiplexed samples that combine molecular marker discovery and genotyping. GBS is a novel application of NGS protocols for discovering and genotyping SNPs in crop genomes and populations. The GBS approach includes the digestion of genomic DNA with restriction enzymes followed by the ligation of barcode adapter, PCR amplification and sequencing of the amplified DNA pool on a single lane of flow cells. Bioinformatic pipelines are needed to analyze and interpret GBS datasets. As an ultimate MAS tool and a cost-effective technique, GBS has been successfully used in implementing genome-wide association study (GWAS), genomic diversity study, genetic linkage analysis, molecular marker discovery and genomic selection under a large scale of plant breeding programs.
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影响因子：
48
作者：
Craig, David W.;Pearson, John V.;Szelinger, Szabolcs;Sekar, Aswin;Redman, Margot;Corneveaux, Jason J.;Pawlowski, Traci L.;Laub, Trisha;Nunn, Gary;Stephan, Dietrich A.;Homer, Nils;Huentelman, Matthew J.
通讯作者：
Huentelman, Matthew J.
影响因子：
4
作者：
Kilian B;Graner A
通讯作者：
Graner A
影响因子：
3.7
作者：
Baird NA;Etter PD;Atwood TS;Currey MC;Shiver AL;Lewis ZA;Selker EU;Cresko WA;Johnson EA
通讯作者：
Johnson EA
影响因子：
3.3
作者：
Beissinger TM;Hirsch CN;Sekhon RS;Foerster JM;Johnson JM;Muttoni G;Vaillancourt B;Buell CR;Kaeppler SM;de Leon N
通讯作者：
de Leon N
影响因子：
4.4
作者：
Chutimanitsakun Y;Nipper RW;Cuesta-Marcos A;Cistué L;Corey A;Filichkina T;Johnson EA;Hayes PM
通讯作者：
Hayes PM