Bacillus subtilis pgsE (Formerly ywtC) Stimulates Poly-γ-Glutamate Production in the Presence of Zinc
Bacillus subtilis pgsE (Formerly ywtC) Stimulates Poly-γ-Glutamate Production in the Presence of Zinc
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DOI：
10.1002/bit.22913
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发表时间：
2011-01-01
影响因子：
3.8
通讯作者：
Ashiuchi, Makoto
中科院分区：
文献类型：
--
作者：
Yamashiro, Daisuke;Yoshioka, Megumi;Ashiuchi, Makoto
Poly-gamma-glutamate (PGA) is a versatile nylon-like material, and enhanced production of PGA is required for various bio-industrial applications. In this study, we first examined the effects of available sugars on the production of Bacillus subtilis PGA, and demonstrated the good applicability of pentoses (e.g., D-xylose). Then, we characterized the pgsE gene of B. subtilis, which encodes a 6.5-kDa protein of 55 amino acids (PgsE), as a genetic tool for increasing the yield of PGA without changing its structural features (e.g., polymer stereochemistry and molecular size distribution). In the presence of Zn2+, the induction of PgsE tripled the PGA productivity of B. subtilis subsp. chungkookjang. This finding will contribute to the establishment of an improved PGA-production system. Biotechnol. Bioeng. 2011;108: 226-230. (C) 2010 Wiley Periodicals, Inc.
