Antibodies differentiate desmosome-form and nucleus-form pinin: Evidence that pinin is a moonlighting protein with dual location at the desmosome and within the nucleus
Antibodies differentiate desmosome-form and nucleus-form pinin: Evidence that pinin is a moonlighting protein with dual location at the desmosome and within the nucleus
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DOI：
10.1006/bbrc.1999.1353
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发表时间：
1999-09-16
影响因子：
3.1
通讯作者：
Pin, OY
中科院分区：
文献类型：
--
作者：
Pin, OY
Pinin 是一种存在于上皮、心肌和脑膜中的桥粒相关蛋白。发现该分子能够增强细胞连接形成，并被认为在上皮细胞中桥粒-中间丝复合物的重组和稳定中发挥关键作用(J, Cell Biol. (1996) 135, 1027-1042)。最近报道了一种声称仅位于细胞核中的蛋白质，其氨基酸序列与pinin相同(E, J. Cell Biol. (1998) 75, 295-298)。在这里，我通过产生位置特异性单克隆抗体来证明 pinin 同时存在于桥粒和细胞核内。尽管 cDNA 文库筛选和 DNA 测序证明，桥粒形式（d 形式）和核形式（n 形式）pinin 具有相同的氨基酸序列，但它们表现出显着不同的生化特性，反映了其不同细胞位置的明显不同的多蛋白性质。此外，d-型pinin的特征在于动态运输过程，该过程涉及核材料的逐渐减少，相对于成熟细胞上pinin与桥粒的锚定增强。最后我证明 pinin 以两种不同的基因产物形式存在：pinin1 和 pinin2。这些数据强烈反对pinin是一种独特的核蛋白的说法，并支持pinin是一种兼职蛋白，由于其双重细胞定位而具有多种功能的观点。 (C) 1999 年学术出版社。
Pinin is a desmosome-associated protein occurring in epithelia, cardiac muscle, and meninges. This molecule was found to be capable of enhancing cell junction formation and thought to play a key role in reorganization and stabilization of the desmosome-intermediate filament complex in epithelial cells (J, Cell Biol. (1996) 135, 1027-1042). Recently a protein, claimed to be localized exclusively in the nucleus, however, with amino acid sequence identical to pinin, was reported (E, J. Cell Biol. (1998) 75, 295-298). Here I present evidence that pinin exists simultaneously at the desmosome and within the nucleus by generating location-specific monoclonal antibodies. Although the desmosome-form (d-form) and the nucleus-form (n-form) pinin share identical amino acid sequences as demonstrated by cDNA library screening and DNA sequencing, they exhibit remarkably different biochemical properties, reflecting the apparent different multiprotein nature of their differential cellular locations. In addition, the d-form pinin is characterized by a dynamic transport process which involves the gradual diminishing of nuclear materials relative to enhanced anchoring of pinin to the desmosome upon mature cells. Finally I demonstrate that pinin exists in two forms of different gene product: pinin1 and pinin2. These data argue strongly against the statement that pinin is an exclusive nuclear protein and support the notion that pinin is a moonlighting protein with more than one function as a consequence of its dual cellular location. (C) 1999 Academic Press.