Anthocyanin-assisted Agrobacterium infiltration for the rapid evaluation of genome editing efficiencies across multiple plant species
Anthocyanin-assisted Agrobacterium infiltration for the rapid evaluation of genome editing efficiencies across multiple plant species
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DOI：
10.1360/nso/20220052
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发表时间：
2022-11
期刊：
影响因子：
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通讯作者：
Sinian Xing;Yu Sun;Boshu Li;Hongchao Li;K. Zhao;Kunling Chen;Caixia Gao
中科院分区：
文献类型：
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作者：
Sinian Xing;Yu Sun;Boshu Li;Hongchao Li;K. Zhao;Kunling Chen;Caixia Gao
：以CRISPR为基础的基因组编辑技术继续推动生命科学的重大进步。实现基因组编辑在植物和农业中的广泛应用的一个主要挑战是建立能够使用瞬变方法对编辑技术进行快速、全面和准确评估的方法。在这里，我们报告了一种新的、快速的基因组编辑评估方法，使用农杆菌渗透技术来实现对基因组编辑效率的广谱、简化和精确的评估。我们使用了花青素标记来促进基因组编辑细胞的可视化筛选，用于成年草莓水果以及番茄水果、棉花叶片和甜菜叶片。使用这种方法，我们展示了快速测量由SpCas9、LbCas12a、A3A-PBE、ABE8e和PPE介导的基因组编辑效率的能力。这一新方法将使研究人员能够快速、轻松地评估广泛植物物种的基因组编辑工具，进一步加快基因组编辑农业作物的发展。
: CRISPR-based genome editing technologies continue to drive major advances in life sciences. A major challenge for realizing widespread use of genome editing in plants and agriculture is establishing methods that enable the rapid, comprehensive, and precise evaluation of editing technologies using transient methods. Here we report a new and rapid genome editing evaluation method using Agrobacterium infiltration techniques to enable broad-spectrum, simplistic, and precise assessments of genome editing efficiencies. We employed an anthocyanin marker to facilitate visual screenings of genome-edited cells for use in adult strawberry fruits as well as tomato fruits, cotton leaves, and sugar beet leaves. Using this method, we demonstrate the ability to quickly measure genome editing efficiencies mediated by SpCas9, LbCas12a, A3A-PBE, ABE8e, and PPE. This new method will allow researchers to rapidly and easily evaluate genome editing tools across a broad spectrum of plant species, further expediting the development of genome-edited agricultural crops.