Ultraviolet-B Radiation Induced Cross-linking Improves Physical Properties of Cold- and Warm-Water Fish Gelatin Gels and Films
Ultraviolet-B Radiation Induced Cross-linking Improves Physical Properties of Cold- and Warm-Water Fish Gelatin Gels and Films
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DOI：
10.1111/j.1750-3841.2012.02839.x
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发表时间：
2012-09-01
影响因子：
3.9
通讯作者：
McHugh, Tara H.
中科院分区：
文献类型：
--
作者：
Otoni, Caio G.;Avena-Bustillos, Roberto J.;McHugh, Tara H.
冷水和温水鱼明胶颗粒暴露于紫外线-B辐射剂量高达29.7 J/cm 2。从颗粒制备溶液和膜。凝胶电泳和折射率用于检查样品的分子量的变化。此外，凝胶强度和流变性能的解决方案，以及拉伸和水蒸气阻隔性能的薄膜进行了表征。SDS-PAGE和折射率结果表明暴露于辐射后明胶链的交联。有趣的是，UV-B处理过的样品显示出更高的凝胶强度，冷水和温水鱼明胶的凝胶强度分别从1.39 N增加到2.11 N和从7.15 N增加到8.34 N。此外，两种明胶样品在较高UV剂量下均显示粘度增加。对于明胶薄膜，冷水鱼明胶样品由辐照颗粒表现出更大的拉伸强度。相比之下，由辐照颗粒制成的温水明胶膜的拉伸强度较低，但水蒸气阻挡性能较好。这可能是由于在温水明胶中UV诱导的交联破坏了螺旋结构。
Cold- and warm-water fish gelatin granules were exposed to ultraviolet-B radiation for doses up to 29.7 J/cm2. Solutions and films were prepared from the granules. Gel electrophoresis and refractive index were used to examine changes in molecular weight of the samples. Also, the gel strength and rheological properties of the solutions as well as the tensile and water vapor barrier properties of the films were characterized. SDS-PAGE and refractive index results indicated cross-linking of gelatin chains after exposure to radiation. Interestingly, UV-B treated samples displayed higher gel strengths, with cold- and warm-water fish gelatin having gel strength increases from 1.39 to 2.11 N and from 7.15 to 8.34 N, respectively. In addition, both gelatin samples exhibited an increase in viscosity for higher UV doses. For gelatin films, the cold-water fish gelatin samples made from irradiated granules showed greater tensile strength. In comparison, the warm-water gelatin films made from irradiated granules had lower tensile strength, but better water vapor barrier properties. This might be due to the UV induced cross-linking in warm-water gelatin that disrupted helical structures.