Temporal Properties of Cone ERGs of Pikachurin Null Mutant Mouse.
Temporal Properties of Cone ERGs of Pikachurin Null Mutant Mouse.
复制标题
皮卡丘林无效突变小鼠视锥 ERG 的时间特性。
DOI：
10.1167/iovs.15-17708
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Terasaki H.
中科院分区：
文献类型：
--
作者：
1.Kominami T;Ueno S;Nakanishi A;Kominami A;Kondo M;Furukawa T;Terasaki H.
目的：Pikachurin是一种位于光感受器突触间隙的细胞外基质样蛋白。Pikachurin零突变（Pika−/−）小鼠具有异常的ON双极细胞功能。本研究旨在确定Pika - / -小鼠的ON双极细胞通路（ON通路）对闪烁ERG反应的贡献，并使用矢量分析，确定其贡献如何随刺激频率变化。方法：记录野生型（WT）、Pika−/−和mGluR6零突变体（mGluR6−/−）小鼠的闪烁ERGs。刺激频率为3.906 ~ 31.250 Hz。通过谐波和矢量模型分析，得到了基元分量的幅值和相位。mGluR6−/−小鼠被用作模型，其中已知不存在ON通路。结果：在3.906 ~ 17.578 Hz的刺激频率范围内，Pika−/−小鼠的基本成分振幅明显小于WT小鼠。Pika - / -小鼠的基本组分相介于WT和mGluR6 - / -小鼠之间。载体分析表明，在低频（即< 15.625 Hz）时，Pika - / -小鼠ON通路的功能是WT小鼠的12% ~ 25%；然而，在17.578赫兹的频率下，它被降低到噪音水平。结论：载体模型分析可以确定Pika - / -小鼠ON通路对闪烁ERG反应的贡献程度，并可用于确定ON通路异常小鼠模型的视网膜功能。
Purpose: Pikachurin is an extracellular matrix-like protein located in the synaptic cleft of photoreceptors. Pikachurin null mutant (Pika−/−) mice have abnormal ON bipolar cell function. This study aimed to determine the contribution of the ON bipolar cell pathway (ON pathway) of Pika−/− mice to the flicker ERG response and, using vector analysis, identify how the contribution varies with the stimulus frequency.Methods: Flicker ERGs were recorded from wild-type (WT), Pika−/−, and mGluR6 null mutant (mGluR6−/−) mice. The frequency of stimulation was 3.906 to 31.250 Hz. The amplitude and phase of the fundamental components were obtained by harmonic and vector model analysis. The mGluR6−/− mice were used as a model wherein the ON pathway is known to be absent.Results: The amplitudes of the fundamental components of the Pika−/− mice were significantly smaller than those of WT mice for stimulation frequencies between 3.906 and 17.578 Hz. The phase of the fundamental components of the Pika−/− mice was between those of the WT and mGluR6−/− mice. Vector analyses showed that the functioning of the ON pathway of Pika−/− mice was 12% to 25% of that of the WT mice at low frequencies (ie,< 15.625 Hz); however, it was reduced to noise level at frequencies> 17.578 Hz.Conclusions: Vector model analysis can determine the degree of contribution of the ON pathway of Pika−/− mice to flicker ERG response and may be useful for determining the retinal function in mice models with abnormalities of the ON pathway.