CLONING BY RECOGNITION SITE SCREENING OF 2 NOVEL GT BOX BINDING-PROTEINS - A FAMILY OF SP1 RELATED GENES
CLONING BY RECOGNITION SITE SCREENING OF 2 NOVEL GT BOX BINDING-PROTEINS - A FAMILY OF SP1 RELATED GENES
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DOI：
10.1093/nar/20.21.5519
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发表时间：
1992-11-11
影响因子：
14.9
通讯作者：
SUSKE, G
中科院分区：
文献类型：
--
作者：
HAGEN, G;MULLER, S;SUSKE, G
先前对子宫珠蛋白基因启动子的分析揭示了一个GT 1盒，该盒也存在于SV 40增强子中。子宫珠蛋白启动子背景下的GT 1元件在人子宫内膜细胞系石川细胞中有活性，但在HeLa细胞中无活性。在这里，我们报告的克隆识别位点筛选的两个因素（SPR-1和SPR-2）结合到这个GT 1基序。SPR-1和SPR-2是转录因子Sp1的同源物。所有这三种蛋白质都是编码具有非常相似结构特征的蛋白质的基因家族的密切相关的成员。与Spl一样，SPR-1和SPR-2含有富含谷氨酰胺和丝氨酸/苏氨酸的氨基酸段。最重要的是，所有三种蛋白质的DNA结合结构域都是高度保守的，它们识别GT以及GC盒相同。SPR-2 mRNA普遍表达，而SPR-1转录本在大脑中丰富，但在其他器官中几乎检测不到。这些因素的子宫珠蛋白启动子的活性的可能功能进行了讨论。
Previous analyses of the uteroglobin gene promoter revealed a GT1 box which is also found in the SV40 enhancer. The GT1 element in the context of the uteroglobin promoter is active in Ishikawa cells, a human endometrial cell line, but not in HeLa cells. Here we report the cloning by recognition site screening of two factors (SPR-1 and SPR-2) which bind to this GT1 motif. SPR-1 and SPR-2 are homologues of the transcription factor Sp1. All three proteins are closely related members of a gene family encoding proteins with very similar structural features. Like Spl, SPR-1 and SPR-2 contain glutamine and serine/threonine rich amino acid stretches. Most significantly, the DNA binding domains of all three proteins are highly conserved and they recognize GT as well as GC boxes identically. SPR-2 mRNA is expressed ubiquitously, whereas SPR-1 transcripts are abundant in the brain but barely detectable in other organs. The possible function of these factors for the activity of the uteroglobin promoter is discussed.