Plasma Mesothelin as a Novel Diagnostic and Prognostic Biomarker in Colorectal Cancer.
Plasma Mesothelin as a Novel Diagnostic and Prognostic Biomarker in Colorectal Cancer.
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血浆间皮素作为结直肠癌的新型诊断和预后生物标志物
DOI：
10.7150/jca.18014
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发表时间：
2017
影响因子：
3.9
通讯作者：
Wang M
中科院分区：
文献类型：
--
作者：
Li S;Xie L;He L;Fan Z;Xu J;Xu K;Zhu L;Ma G;Du M;Chu H;Zhang Z;Ni M;Wang M
目的间皮蛋白是一种细胞表面蛋白，在多种肿瘤中均有过表达。然而，间硫蛋白作为血浆生物标记物在结直肠癌中的潜在价值还没有被探索。本研究的目的是确定血浆间充质蛋白是否适合作为结直肠癌的诊断和预后生物标记物。方法采用双抗体夹心酶联免疫吸附试验(EL ISA)对结直肠癌患者进行两阶段病例对照研究。分别于术前、术后采集血浆，观察手术对血浆水平的影响。应用受试者工作特征(ROC)曲线评价血浆间充质蛋白的诊断价值。并对有生存信息的患者进行单因素Kaplan-Meier生存分析和Cox回归分析。结果发现结直肠癌患者血浆中的间充质蛋白水平显著高于对照组(P<；0.001)，其AUC值为0.690(95%CI=0.625~0.752)。低水平患者的生存时间较长(校正后HR=4.43，95%CI=1.93~10.15，P<；0.001)。此外，患者术后血浆中间皮质素水平较术前略有下降，但差异无统计学意义(P=0.052)。结论血浆间皮蛋白水平与结直肠癌的发生、发展密切相关。血浆间硫蛋白可能是结直肠癌潜在的诊断和/或预后的生物标志物。
Objective Mesothelin is a cell surface protein and overexpressed in many cancers. However, the potential value of mesothelin as plasma biomarker in colorectal cancer has not been explored. The purpose of this study was to identify whether plasma mesothelin is a suitable diagnostic and prognostic biomarker for colorectal cancer. Methods We performed a two-stage case-control study to evaluate plasma mesothelin levels in colorectal cancer using enzyme-linked immunosorbent assay (ELISA). Preoperative and postoperative plasma were collected to examine the level changes influenced by surgery. Receiver operating characteristic (ROC) curves were applied to identify the diagnostic value of plasma mesothelin. We also conducted univariate Kaplan-Meier survival analysis and Cox regression analysis of patients with survival information. Results We found that the plasma mesothelin levels in colorectal cancer patients were significantly higher than that in the controls (P < 0.001) with an AUC value of 0.690 (95% CI = 0.625 to 0.752). Individuals with lower mesothelin level had a longer survival time (adjusted HR = 4.43, 95% CI = 1.93-10.15, P < 0.001). Furthermore, Patients had slightly decreased mesothelin levels in postoperative plasma than preoperative plasma, although the alteration was not statistically significant (P = 0.052). Conclusion Our findings highlight the correlative relationship between plasma mesothelin levels and the presence and progression of colorectal cancer. Plasma mesothelin may be a potential diagnostic and, or prognostic biomarker for colorectal cancer.