PROLIFERATIVE CAPACITY AND DNA CONTENT OF AGING HUMAN DIPLOID CELLS IN CULTURE - CYTOPHOTOMETRIC AND AUTORADIOGRAPHIC ANALYSIS
PROLIFERATIVE CAPACITY AND DNA CONTENT OF AGING HUMAN DIPLOID CELLS IN CULTURE - CYTOPHOTOMETRIC AND AUTORADIOGRAPHIC ANALYSIS
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DOI：
10.1002/jcp.1040840202
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发表时间：
1974-01-01
影响因子：
5.6
通讯作者：
CRISTOFALO, VJ
中科院分区：
文献类型：
--
作者：
YANISHEVSKY, R;MENDELSOHN, ML;CRISTOFALO, VJ
结合DNA细胞光度法和氚化胸苷放射自显影法，我们已经证明，在连续繁殖的人二倍体细胞群中，大多数非分裂细胞的2C DNA含量与其在二倍体细胞周期的G1期被阻滞一致。未标记的4C细胞在培养中随着时间的推移而增加。这些细胞可能是G2二倍体，也可能是G1四倍体，因为在较老的培养物中，多倍体的增加与此相关，这可以通过标记的8C细胞的出现来证明。
Using a combination of DNA‐cytophotometry and tritiated thymidine‐autoradiography, we have shown that the majority of nondividing cells in serially propagated human diploid cell populations have the 2C DNA content consistent with their being arrested in the G1phase of the diploid cell cycle. Unlabeled 4C cells appear increasingly with time in culture. These may be arrested G2diploids or they may be G1tetraploids, since there is an associated increase in polyploidy in older cultures as evidenced by the appearance of labeled 8C cells.