Natural variation analysis of perennial ryegrass in response to abiotic stress highlights LpHSFC1b as a positive regulator of heat stress
Natural variation analysis of perennial ryegrass in response to abiotic stress highlights LpHSFC1b as a positive regulator of heat stress
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多年生黑麦草响应非生物胁迫的自然变异分析强调 LpHSFC1b 作为热应激的正调节因子
DOI：
10.1016/j.envexpbot.2020.104192
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发表时间：
2020-11-01
影响因子：
5.7
通讯作者：
Chan, Zhulong
中科院分区：
文献类型：
--
作者：
Sun, Tianxiao;Shao, Kun;Chan, Zhulong
多年生黑麦草（Lolium perenne L.）是世界各地栽培的冷季饲料和草坪草品种。多年生黑麦草品种的胁迫耐受性的自然变化和胁迫耐受机制引起了育种者的极大兴趣。在本研究中，对 17 个品种的胁迫耐受性和 HSFC1b 的功能进行了表征。结果显示，17 个品种对干旱、热和冷胁迫表现出不同的耐受性。胁迫处理后，耐受品种的脯氨酸含量高于敏感品种。干旱、热和冷胁迫对多年生黑麦草的转录组进行了广泛的重编程，并分别调节了 4797、4329 和 4635 个基因的表达。 LpHSFC1b 在应激处理后显着诱导，并通过蛋白质相互作用网络分析表征为核心调节因子。拟南芥中 LpHSFC1b 的异位表达改善了植物的耐热性，但没有改善干旱和寒冷的耐受性。转基因拟南芥降低了电解质渗漏（EL）、丙二醛（MDA）含量和失绿前斜线坏死严重程度指数，并增加了热应激反应基因的表达。这些数据表明，多年生黑麦草表现出不同的胁迫耐受性，HSFC1b可能在热应激反应中发挥正向调节作用，这可能为了解多年生黑麦草的非生物胁迫反应线索提供详细的见解。
Perennial ryegrass (Lolium perenne L.) is a cool-season forage and turf grass species cultivated world-wide. Natural variations of stress tolerance of perennial ryegrass varieties and mechanisms of stress tolerance are of great interest for breeders. In this study, stress tolerances of seventeen varieties and function of HSFC1b have been characterized. The results showed seventeen varieties exhibited contrasting tolerance to drought, heat and cold stresses. Tolerant varieties had higher proline than sensitive ones after stress treatments. Drought, heat and cold stresses extensively reprogrammed transcriptome of perennial ryegrass and modulated expression of 4797, 4329 and 4635 genes, respectively. LpHSFC1b was significantly induced after stress treatments and characterized as the core regulators through protein interaction network analysis. Ectopic expression of LpHSFC1b in Arabidopsis improved plant thermotolerance, but not drought and cold tolerances. Transgenic Arabidopsis had decreased electrolyte leakage (EL), malondialdehyde (MDA) content and chlorosis forward slash necrosis severity indices, and increased expression of heat stress responsive genes. These data indicated that perennial ryegrass exhibited divergent stress tolerances, and HSFC1b likely functioned as a positive regulator in heat stress response, that may provide detailed insight for understanding of abiotic stress response clues in perennial ryegrass.