SOME PRACTICAL CONSIDERATIONS AND APPLICATIONS OF THE NATIONAL-CANCER-INSTITUTE IN-VITRO ANTICANCER DRUG DISCOVERY SCREEN
SOME PRACTICAL CONSIDERATIONS AND APPLICATIONS OF THE NATIONAL-CANCER-INSTITUTE IN-VITRO ANTICANCER DRUG DISCOVERY SCREEN
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DOI：
10.1002/ddr.430340203
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发表时间：
1995-02-01
影响因子：
3.8
通讯作者：
PAULI, KD
中科院分区：
文献类型：
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作者：
BOYD, MR;PAULI, KD
在1985-1990年期间，美国国家癌症研究所（NCl）逐步取消了其小鼠白血病P388抗癌药物筛选计划，并开发了一种基于多种人类肿瘤细胞系的新的体外初步筛选作为替代。对于每种测试的物质，该筛选在构成该组的60种不同细胞系中产生差异性体外细胞敏感性或缺乏差异性体外细胞敏感性的显著可再现和特征性概况。已经探索了几种研究方法来显示，分析和解释这些配置文件和数据库，这些配置文件和数据库来自于自NCl屏幕全面运行以来的过去4-5年中对数万种物质的测试。体外筛选的各种有用的实际应用已经变得显而易见。随着这些应用的不断发展，它们被证明是对其他地方正在开发或追求的各种其他抗癌筛选和药物发现策略的补充。本文综述了一些实际考虑和选定的具体实例，特别是说明了NCl筛选的研究应用，其可更广泛地适用于寻找具有抗肿瘤活性和/或作用机制的新抗癌药物开发线索。(C)1995 Wiley-Liss，Inc.
During 1985-1990 the U.S. National Cancer Institute (NCl) phased out its murine leukemia P388 anticancer drug screening program and developed as the replacement a new in vitro primary screen based upon a diverse panel of human tumor cell lines. For each substance tested, the screen generates a remarkably reproducible and characteristic profile of differential in vitro cellular sensitivity, or lack thereof, across the 60 different cell lines comprising the panel. Several investigational approaches to display, analysis, and interpretation of such profiles and databases, derived from the testing of tens of thousands of substances during the past 4-5 years since the NCl screen became fully operational, have been explored. A variety of useful, practical applications of the in vitro screen have become apparent. As these applications continue to evolve, they are proving to be complementary to diverse other anticancer screening and drug discovery strategies being developed or pursued elsewhere. Reviewed herein are some practical considerations and selected specific examples, particularly illustrating research applications of the NCl screen that may be more broadly applicable to the search for new anticancer drug development leads with novel profiles of antitumor activity and/or mechanisms of action. (C) 1995 Wiley-Liss, Inc.