Characterization of the importance of polysaccharide intercellular adhesin/hemagglutinin of Staphylococcus epidermidis in the pathogenesis of biomaterial-based infection in a mouse foreign body infection model
Characterization of the importance of polysaccharide intercellular adhesin/hemagglutinin of Staphylococcus epidermidis in the pathogenesis of biomaterial-based infection in a mouse foreign body infection model
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DOI：
10.1128/iai.67.5.2627-2632.1999
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发表时间：
1999-05-01
影响因子：
3.1
通讯作者：
Mack, D
中科院分区：
文献类型：
--
作者：
Rupp, ME;Ulphani, JS;Mack, D
生物膜的产生被认为在表皮葡萄球菌引起的假体装置感染的发病机制中至关重要。使用实验动物模型来评估生物膜产生的重要性，生物膜的产生由多糖细胞间粘附素/血凝素（PIA/HA）介导，在基于生物材料的感染的发病机制中。沿皮下植入的静脉导管的长度给小鼠接种野生型 S、表皮 1457 或其同基因 PIA/HA 阴性突变体。野生型菌株比突变型菌株更容易引起皮下脓肿（P < 0.01），并且通过宿主防御从接种位点根除的可能性也显着降低（P < 0.05）。此外，发现野生型菌株比 PIG/HA 阴性突变体更丰富地粘附到植入的导管上（P < 0.05），通过扫描电子显微镜评估粘附测定的可靠性。为了排除污染或自发感染，通过脉冲场凝胶电泳分析限制性片段长度多态性模式，将从实验动物中回收的细菌菌株与接种菌株进行比较。野生型菌株及其同基因突变体与纤连蛋白包被表面的体外结合是相似的。这些结果证实了由 PIA/HA 介导的生物膜产生在表皮葡萄球菌实验性异物感染发病机制中的重要性。
The production of biofilm is thought to be crucial in the pathogenesis of prosthetic-device infections caused by Staphylococcus epidermidis, An experimental animal model was used to assess the importance of biofilm production, which is mediated by polysaccharide intercellular adhesin/hemagglutinin (PIA/HA), in the pathogenesis of a biomaterial-based infection. Mice were inoculated along the length of a subcutaneously implanted intravenous catheter with either wild-type S, epidermidis 1457 or its isogenic PIA/HA-negative mutant. The wild-type strain was significantly more likely to cause a subcutaneous abscess than the mutant strain (P < 0.01) and was significantly less likely to be eradicated from the inoculation site by host defense (P < 0.05). In addition, the wild-type strain was found to adhere to the implanted catheters more abundantly than the PIG/HA-negative mutant (P < 0.05), The reliability of the adherence assay was assessed by scanning electron microscopy, To exclude contamination or spontaneous infection, bacterial strains recovered from the experimental animals were compared to inoculation strains by analysis of restriction fragment length polymorphism patterns by pulsed-field gel electrophoresis. In vitro binding of the wild-type strain and its isogenic mutant to a fibronectin-coated surface was similar. These results confirm the importance of biofilm production, mediated by PIA/HA, in the pathogenesis of S, epidermidis experimental foreign body infection.