Infection and Maintenance of Holospora Species in Paramecium caudatum
Infection and Maintenance of Holospora Species in Paramecium caudatum
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DOI：
10.1007/978-3-540-92677-1_8
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发表时间：
2009-01-01
期刊：
影响因子：
--
通讯作者：
Fujishima, Masahiro
中科院分区：
文献类型：
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作者：
Fujishima, Masahiro
全孢霉属的感染形式有一个细胞质区和一个周质腔，周质腔有一个电子半透明的侵入尖端。细菌89-kDa的蛋白质与两个肌动蛋白结合基序易位时，感染形式被摄入的草履虫细胞的消化泡从入侵端腔外的提示。利用侵入尖端，细菌破坏消化泡膜，出现在宿主细胞质中，迁移到其靶核，通过外膜的细菌脂多糖与靶核膜的未知物质之间的亲和力区分两种宿主核膜，然后利用侵入尖端穿透靶核膜。第一个细菌在混合后10分钟内侵入宿主细胞核。内共生后，细菌改变宿主基因表达，为阐明真核细胞和原核细胞之间的宿主-共生体相互作用以及建立导致真核细胞进化的新关联提供了极好的机会。
Infectious forms of Holospora species have a cytoplasmic region and a periplasmic lumen with an electron-translucent invasion tip. Bacterial 89-kDa proteins with two actin-binding motifs translocate from the invasion tip lumen to outside the tip when infectious forms are ingested in digestive vacuoles of a Paramecium cell. With the invasion tip, the bacteria disrupt the digestive vacuole membrane, appear in the host cytoplasm, migrate to their target nucleus, distinguish host nuclear envelopes of two kinds through affinity between the bacterial lipopolysaccharides of the outer membranes and an unknown substance of the target nuclear envelope, and then penetrate the target nuclear envelope with the invasion tip. The first bacterium invades the host nucleus within 10 min after mixing. After endosymbiosis the bacteria alter host gene expression, providing an excellent opportunity for elucidating host-symbiont interactions between eukaryotic and prokaryotic cells and establishment of new associations leading to eukaryotic cell evolution.