Serologic Survey of Mosquito-Borne Viruses in Hunter-Harvested White-Tailed Deer (Odocoileus virginianus), New York State
Serologic Survey of Mosquito-Borne Viruses in Hunter-Harvested White-Tailed Deer (Odocoileus virginianus), New York State
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DOI：
10.4269/ajtmh.20-1090
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发表时间：
2021-02-01
影响因子：
3.3
通讯作者：
Kramer, Laura D.
中科院分区：
文献类型：
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作者：
Dupuis, Alan P.;Prusinski, Melissa A.;Kramer, Laura D.
2007-2015 年在纽约州 (NYS) 狩猎采集的白尾鹿（WTD，Odocoileus virginianus）血清通过噬菌斑减少中和法进行了检测，以检测针对周布尼亚病毒科、黄病毒科和披膜病毒科的九种蚊媒病毒的抗体。总体而言，76.1% (373/490) 的 WTD 样本对至少一种病毒呈血清阳性，38.8% 暴露于多种病毒。成年WTD血清阳性率（78.0%）显着高于小鹿（47.7%）（P < 0.0001）。在所有地区采样的 WTD 中，针对加州血清群病毒的中和抗体最常见 (67.1%)，其次是 Bunyamwera 血清群 (BUN) (37.6%)。詹姆斯敦峡谷 (Jamestown Canyon) 和卡什谷 (Cache Valley) 正布尼亚病毒分别导致了大多数加州和 BUN 感染。来自长岛 (LI) 的样本中西尼罗河病毒的血清阳性率 (19.0%) 高于来自纽约州中部 (7.3%)、西部 (5.0%) 和哈德逊河谷 (4.4%) 地区的样本。东部马脑炎病毒抗体主要见于纽约州中部的 WTD（5.1%），那里每年都有地方性动物流行活动，但纽约州西部（1.4%）和 LI（1.7%）的比率较低。在本次调查过程中，检测到波托西病毒、曲棍球正布尼亚病毒以及高地 J 病毒抗体的比例较低。在纽约州中部和西部收集的样本中检测到了圣路易斯脑炎病毒（或密切相关的病毒）抗体，这表明尽管缺乏常规蚊子监测的证据，但当地存在病毒传播。血清学结果证明了纽约州 WTD 作为虫媒病毒分布和近期在相对精细的空间尺度上传播的指标的价值。
Sera from white-tailed deer (WTD, Odocoileus virginianus) hunter-harvested throughout New York State (NYS), 2007-2015, were tested by plaque reduction neutralization for antibodies against nine mosquito-borne viruses from the families Peribunyaviridae, Flaviviridae, and Togaviridae. Overall, 76.1% (373/490) of sampled WTD were seropositive against at least one virus, and 38.8% were exposed to multiple viruses. The seropositivity rate in adult WTD (78.0%) was significantly greater (P < 0.0001) than that in fawns (47.7%). Neutralizing antibodies against California serogroup viruses were most common in WTD sampled across all regions (67.1%), followed by the Bunyamwera serogroup (BUN) (37.6%). Jamestown Canyon and Cache Valley orthobunyaviruses were responsible for most California and BUN infections, respectively. Seroprevalence rates to West Nile virus were higher in samples originating from Long Island (LI) (19.0%) than in those originating from the central (7.3%), western (5.0%), and Hudson Valley (4.4%) regions of NYS. Antibodies to Eastern equine encephalitis virus were seen primarily in WTD from central NYS (5.1%), where annual enzootic activity occurs, but low rates were documented in western NYS (1.4%) and LI (1.7%). Low rates of Potosi and LaCrosse orthobunyavirus, and Highlands J virus antibodies were detected over the course of this investigation. St. Louis encephalitis virus (or a closely related virus) antibodies were detected in samples collected from central and western NYS, suggesting local virus transmission despite a lack of evidence from routine mosquito surveillance. Serologic results demonstrate the value of WTD in NYS as an indicator of arbovirus distribution and recent transmission on a relatively fine spatial scale.