Bacterial susceptibility to and chemical composition of essential oils from Thymus kotschyanus and Thymus persicus
Bacterial susceptibility to and chemical composition of essential oils from Thymus kotschyanus and Thymus persicus
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DOI：
10.1021/jf0261755
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发表时间：
2003-04-09
影响因子：
6.1
通讯作者：
Mirmostafa, SA
中科院分区：
文献类型：
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作者：
Rasooli, I;Mirmostafa, SA
大肠埃希菌、金黄色葡萄球菌、枯草芽孢杆菌、肺炎克雷伯菌和铜绿假单胞菌对两种百里香精油的敏感性。还有霍恩。和百合在开花前和开花期进行了比较研究。采用纸片扩散法评价了不同浓度油品对微生物生长的抑制区。测定了它们的最低抑菌浓度和最低杀菌浓度，并进行了比较。从上述植物中提取的精油具有很强的杀菌作用，其中黄曲霉的杀菌效果更好。通过气相色谱(GC)和气质联用(MS)分析，分别鉴定出33种成分和26种成分。除浓度不同外，几乎所有化合物中桃红假单胞菌的挥发油成分与黑曲霉的相似。红豆杉开花前和开花期挥发油的主要成分分别为香芹酚(35.06，22.75%)、百里香酚(26.60，16.52%)、γ-松油烯(7.81，0.34%)、γ-松油烯(4.34，0%)、冰片(2.29，4.52%)、月桂烯(0.26，12.65%)、百里香酚(0，11.39%)、橙花酚(0，6.10%)、β-石竹烯(0，5.54%)。不同虫态的百里香酚分别为香芹酚(38.96%，27.07%)、百里香酚(6.48%，11.86%)、对伞花烃(7.51%，10.16%)、γ-松油醇(0，9.51%)、橙花醇(15.66%，9.41%)、γ-松油烯(6.11%，6.51%)、百里香酚醋酸酯(5.29%，5.30%)。讨论了油的组成对其抗菌性能的贡献。富酚油的芳香族化合物含量高似乎说明其具有较强的抗菌活性。
Susceptibility of Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Klebsiella pneumonia, and Pseudomonas aeroginosa to the essential oils extracted from two varieties of Thyme, i.e., Thymus kotschyanus Boiss. and Hohen. and Thymus persicus L. at preflowering and flowering stages were studied. The disk diffusion method was used to evaluate the zone of microbial growth inhibition at various concentrations of the oils. Minimal inhibitory concentration and minimal bactericidal concentration of the oils were determined and compared with each other. The oils from the above plants were found to be strongly bactericidal with that of T. kotschyanus being more effective. T. kotschyanus and T. persicus oils analyzed by gas chromatography (GC) and GC/mass spectrometry (MS) lead to identification of 33 and 26 components, respectively. The profile of the oil components from T. persicus was similar to that of T. kotschyanus in almost all of the compounds but at different concentrations. The major components of T. kotschyanus oil before and at the flowering stages were carvacrol (35.06, 22.75%), thymol (26.60, 16.52%), gamma-terpinene (7.81, 0.34%), gamma-terpinene (4.34, 0%), borneol (2.29, 4.52%), myrcene (0.26, 12.65%), thymolquinone (0, 11.39%), nerol (0, 6.10%), and beta-caryophyllene (0, 5.54%), respectively, and those of T. persicus at the same stages were carvacrol (38.96, 27.07%), thymol (6.48, 11.86%), P-cymene (7.51, 10.16%), gamma-terpineol (0, 9.51%), nerol (15.66, 9.41%), gamma-terpinene (6.11, 6.51%), and thymol acetate (5.29, 5.30%), respectively. The contribution of oil components to its antibacterial property is discussed. High aromatic compound content of the phenol-rich oils seems to account for strong antibacterial activity.