The role of exosomes derived from cerebrospinal fluid of spinal cord injury in neuron proliferation in vitro
The role of exosomes derived from cerebrospinal fluid of spinal cord injury in neuron proliferation in vitro
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DOI：
10.1080/21691401.2017.1304408
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发表时间：
2018-01-01
影响因子：
5.8
通讯作者：
Wang, Hai-Xu
中科院分区：
文献类型：
--
作者：
Kong, Fan-Long;Wang, Xiao-Ping;Wang, Hai-Xu
外泌体是细胞旁分泌的重要组成部分，在多种疾病模型中发挥重要作用。然而，外泌体在脊髓损伤（SCI）神经元修复中的作用鲜有报道。本研究从脊髓损伤和正常人脑脊液中分离外泌体，分别体外培养神经元，研究其在细胞增殖中的生物学功能。结果表明，这些外泌体均表达CD9、CD63、CD81、Alix和Tsg101；然而，只有脊髓损伤脑脊液来源的外泌体能够通过ERK信号通路促进神经元增殖，减少细胞凋亡。外泌体含有细胞质内容物，包括蛋白质、mrna和非编码rna，并在重要的生物学功能中发挥作用。我们的研究表明脊髓损伤脑脊液外泌体在体外对神经元细胞增殖有影响，而在正常脑脊液外泌体中没有观察到这些特征。
Exosomes is a key component of cell paracrine secretion and can exert important effects in various disease models. However, the role of exosomes in neuron repair of spinal cord injury (SCI) has rarely been reported. In this study, Exosomes were isolated from cerebrospinal fluid of SCI and normal, and incubated neuron in vitro respectively to research its biological function in cell proliferation. The results demonstrated these exosomes all expressed CD9, CD63, CD81, Alix and Tsg101; however, only exosomes derived from cerebrospinal fluid of SCI could promote proliferation of neuron via ERK signaling pathway, and decrease cell apoptosis. Exosomes contain cytosolic content, including proteins, mRNAs and non-cording RNAs, and play a role in important biological function. Our research showed exosomes derived from cerebrospinal fluid of SCI, they can influence neuron cell proliferation in vitro, we did not observe these characters in exosome derived from normal cerebrospinal fluid.