VvCO and VvCOL1, two CONSTANS homologous genes, are regulated during flower induction and dormancy in grapevine buds
VvCO and VvCOL1, two CONSTANS homologous genes, are regulated during flower induction and dormancy in grapevine buds
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DOI：
10.1007/s00299-009-0720-4
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发表时间：
2009-08-01
影响因子：
6.2
通讯作者：
Gonzalez Villanueva, Enrique
中科院分区：
文献类型：
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作者：
Almada, Ruben;Cabrera, Nuri;Gonzalez Villanueva, Enrique
鉴定了两个以前未鉴定的葡萄属酿酒酵母CONSTANS样基因（VvCO，VvCOL1），它们被预测编码与酿酒酵母CONSTANS家族成员同源的蛋白质。在受控条件下，两个基因均显示出在黎明时达到峰值的昼夜表达模式。在葡萄藤芽发育过程中，VvCOL1主要在休眠期表达，表明其参与了葡萄芽休眠诱导和维持的转录光周期调控。另一方面，VvCO在潜伏芽中的表达与开花诱导过程中的功能一致。观察到VvCO、VFY和VvMADS8（拟南芥叶和CONSTANS 1同源物过表达的提供者）在潜伏芽中表达的时空关系，表明这些基因参与葡萄开花的季节性周期。此外，我们的研究结果提供了一个新的分子洞察卷须的发展表明，葡萄藤CO同系物也表达在这个独特的器官。
Two previously uncharacterized Vitis vinifera CONSTANS-like genes (VvCO, VvCOL1), which are predicted to encode proteins with homology to members of the Arabidopis CONSTANS family, were identified. Under controlled conditions, both genes show a diurnal expression pattern with peak at dawn. During grapevine bud development, VvCOL1 is mainly expressed in dormancy, suggesting a participation in the transcriptional photoperiod control of bud dormancy induction and maintenance in this species. On the other hand, VvCO expression in latent buds is in agreement with a function during flowering induction. A spatial and temporal relationship in the expression of VvCO, VFY and VvMADS8 (the Arabidopsis LEAFY and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 orthologues) in latent buds is observed, suggesting that these genes are involved in the seasonal periodicity of flowering in grapevines. Furthermore, our results provide a new molecular insight into tendril development showing that grapevine CO homologues are also expressed in this distinctive organ.