Type C virus in lymphosarcoma in northern pike (Esox lucius)
Type C virus in lymphosarcoma in northern pike (Esox lucius)
复制标题
北梭鱼淋巴肉瘤中的 C 型病毒 (Esox lucius)
作者：
T. Papas;J. Dahlberg;R. Sonstegard
北方狗鱼(Esox Lucius)是一种非常珍贵的淡水鱼，既是一种食用鱼，也是一种商业物种。流行性淋巴肉瘤广泛发生在北美狗鱼1和旧大陆2，3。狗鱼肿瘤的总发生率为20.9%，是已知的自由生活脊椎动物中最高的恶性肿瘤1。所有患有肿瘤的梭子鱼都有皮肤损伤，动物流行病学证据表明，这种疾病在产卵期间通过接触水平传播。肿瘤是可移植的，并且发现了无细胞传播的证据1，4。为了解决这种疾病的病因学，我们调查了梭鱼淋巴瘤中肿瘤病毒的存在。由于所有已知的RNA肿瘤病毒都具有酶逆转录酶，我们从梭鱼淋巴瘤组织制备的线粒体后颗粒组分中寻找这种酶的活性。
THE northern pike (Esox lucius) is a highly prized freshwater fish, both as a game fish and as a commerical species. Epizootics of lymphosarcoma occur widely in North American pike1 and in the Old World2,3. The tumour in pike has been found with an overall frequency of 20.9%, which is the highest frequency of a malignant neoplasm in any known free living vertebrate1. All pike with the tumour have cutaneous lesions, and epizootiological evidence suggests that the disease is transmitted horizontally by contact during spawning1. The tumour is transplantable and evidence of cell-free transmission has been found1,4. In an attempt to resolve the aetiology of the disease, we have investigated the presence of oncornavirus in pike lymphoma. Since all known RNA tumour viruses possess the enzyme reverse transcriptase, we have sought the activity of this enzyme in post-mitochondrial particulate fractions prepared from pike lymphoma tissue.