Characterization of ductular hepatocytes in end-stage cirrhosis.
Characterization of ductular hepatocytes in end-stage cirrhosis.
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发表时间：
1990-04
影响因子：
4.6
通讯作者：
V. Am;J. Burchette;G. Michalopoulos
中科院分区：
文献类型：
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作者：
V. Am;J. Burchette;G. Michalopoulos
根据对大鼠肝脏癌变模型的观察，主张肝脏中存在兼性干细胞。对来自暴发性肝炎病例的人肝脏材料的观察表明存在表达肝细胞和胆管细胞标志物的管状肝细胞。我们描述了一个由B型肝炎感染和继发性胆汁性肝硬化引起的终末期肝硬化患者的导管状肝细胞的形态学特征和抗原表达。通过常规的光学显微镜和电子显微镜观察，在门静脉周围区域内可见导管状肝细胞形成假导管。使用免疫组织化学方法，发现这些小管肝细胞对B型肝炎表面抗原和胆管上皮细胞角蛋白均呈阳性，表型标志物分别经典地局限于肝细胞和胆管上皮上的表达。这些结果明确表明，胆管肝细胞是中间细胞，具有肝细胞和胆管上皮的形态和表型特征。这些细胞的存在表明在成年哺乳动物肝脏中存在兼性干细胞。
The existence of facultative stem cells in the liver has been advocated based on observations from models of carcinogenesis in rat liver. Observations of human liver material from cases of fulminant hepatitis have shown the presence of ductular hepatocytes expressing markers of both hepatocytes and bile duct cells. We describe the morphologic features and antigenic expression of a population of ductular hepatocytes identified in a patient with end-stage cirrhosis resulting from hepatitis B infection and secondary biliary cirrhosis. By conventional light microscopy and electron microscopy, ductular hepatocytes were seen to form pseudoductules within periportal areas. Using immunohistochemical methods, these ductular hepatocytes were found to be positive for both the hepatitis B surface antigen and bile duct epithelial cytokeratin, phenotypic markers classically restricted to expression on hepatocytes and bile duct epithelium, respectively. These findings show definitively that ductular hepatocytes are intermediate cells bearing morphologic and phenotypic characteristics of both hepatocytes and bile duct epithelium. The presence of these cells indicates the existence of facultative stem cells in the adult mammalian liver.