Remote Tuning of Built-In Magnetoelectric Microenvironment to Promote Bone Regeneration by Modulating Cellular Exposure to Arginylglycylaspartic Acid Peptide
Remote Tuning of Built-In Magnetoelectric Microenvironment to Promote Bone Regeneration by Modulating Cellular Exposure to Arginylglycylaspartic Acid Peptide
复制标题
远程调节内置磁电微环境，通过调节细胞对精氨酰甘氨酰天冬氨酸肽的暴露来促进骨再生
DOI：
10.1002/adfm.202006226
复制
发表时间：
2020-11-04
影响因子：
19
通讯作者：
Deng, Xuliang
中科院分区：
文献类型：
--
作者：
Liu, Wenwen;Zhang, Fengyi;Deng, Xuliang
模仿内源性物理微环境是生物材料介导的组织再生的一种有前途的策略。然而，精确控制细胞外环境中的物理信号（例如电场/磁场）以促进组织再生仍然是一个艰巨的挑战。在这里，通过内置的CoFe2O4/聚（偏二氟乙烯三氟乙烯）[P(VDF-TrFE)]磁电膜实现磁电微环境的远程调节，以实现有效的骨再生。正如分子动力学模拟所预测的那样，纳米复合膜的磁电微环境可促进骨髓间充质干细胞（BM-MSC）的成骨分化，并通过增加细胞暴露和整合素与精氨酰甘氨酰天冬氨酸肽的结合来增强骨缺损再生。此外，BM-MSC 通过骨免疫调节定向至成骨谱系，这涉及加速从初始炎症免疫反应到促愈合再生免疫反应的转变。这项工作提供了一种模拟磁电微环境的策略，以实现精确有效的组织再生治疗，并提供了对内置磁电膜驱动的生物效应的基本见解，该膜可以远程调节以精确调节原位成骨。
Mimicking the endogenous physical microenvironment is a promising strategy for biomaterial-mediated tissue regeneration. However, precise control of physical cues such as electric/magnetic fields within extracellular environments to facilitate tissue regeneration remains a formidable challenge. Here, remote tuning of the magnetoelectric microenvironment is achieved by a built-in CoFe2O4/poly(vinylidene fluoridetrifluoroethylene) [P(VDF-TrFE)] magnetoelectric membrane for effective bone regeneration. The magnetoelectric microenvironment from the nanocomposite membranes promotes osteogenic differentiation of bone marrow mesenchymal stem cells (BM-MSCs) and enhances bone defect regeneration by increasing cellular exposure and integrin binding to arginylglycylaspartic acid peptide, as predicted by molecular dynamics simulations. Moreover, BM-MSCs are directed to the osteogenic lineage by osteoimmuomodulation which involves accelerating transition from an initial inflammatory immune response to a pro-healing regenerative immune response. This work offers a strategy to mimic the magnetoelectric microenvironment for achieving precise and effective tissue regenerative therapies, as well as provides fundamental insights into the biological effects driven by the built-in magnetoelectric membrane, which can be remotely tuned to precisely modulate osteogenesis in situ.