Stress relaxation in epithelial monolayers is controlled by actomyosin
Stress relaxation in epithelial monolayers is controlled by actomyosin
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DOI：
10.1101/302158
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发表时间：
2018-04
期刊：
影响因子：
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通讯作者：
N. Khalilgharibi;J. Fouchard;N. Asadipour;Amina Y. Yonis;A. Harris;Payman Mosaffa;Yasuyuki Fujita;A. Kabla;B. Baum;J. Muñoz;M. Miodownik;G. Charras
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文献类型：
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作者：
N. Khalilgharibi;J. Fouchard;N. Asadipour;Amina Y. Yonis;A. Harris;Payman Mosaffa;Yasuyuki Fujita;A. Kabla;B. Baum;J. Muñoz;M. Miodownik;G. Charras
上皮细胞单层是一个细胞厚的组织片，分隔内部和外部环境。作为其功能的一部分，它们承受在高应变率下施加的外部机械应力。然而，很少有人知道单分子膜如何响应机械变形。在应力松弛试验中，单层以双相方式响应，并且应力消散伴随着单层静息长度的增加，这表明在松弛期间细胞结构的主动重塑。与此一致，肌动球蛋白以与机械松弛相称的速率重塑，并控制单层应力松弛的速率-如在单细胞中。相比之下，连接复合物和中间纤维在细胞之间形成稳定的连接，使单层细胞能够像单细胞一样流变。总之，这些数据表明肌动球蛋白细胞骨架动力学通过激活静息长度的ATP依赖性变化来控制单层的流变特性。这些发现对我们理解发育形态发生和组织对机械应力的反应具有深远的影响。
Epithelial monolayers are one-cell thick tissue sheets that separate internal and external environments. As part of their function, they withstand extrinsic mechanical stresses applied at high strain rate. However, little is known about how monolayers respond to mechanical deformations. In stress relaxation tests, monolayers respond in a biphasic manner and stress dissipation is accompanied by an increase in monolayer resting length, pointing to active remodelling of cell architecture during relaxation. Consistent with this, actomyosin remodels at a rate commensurate with mechanical relaxation and governs the rate of monolayer stress relaxation – as in single cells. By contrast, junctional complexes and intermediate filaments form stable connections between cells, enabling monolayers to behave rheologically as single cells. Together, these data show actomyosin cytoskeletal dynamics govern the rheological properties of monolayers by enabling active, ATP-dependent changes in the resting length. These findings have far-reaching consequences for our understanding of developmental morphogenesis and tissue response to mechanical stress.