Assessing the stability of historical and desiccated snake venoms from a medically important Ecuadorian collection
Assessing the stability of historical and desiccated snake venoms from a medically important Ecuadorian collection
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DOI：
10.1016/j.cbpc.2020.108702
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发表时间：
2020-04-01
影响因子：
3.9
通讯作者：
da Silva, Saulo L.
中科院分区：
文献类型：
--
作者：
Almeida, Jose R.;Mendes, Bruno;da Silva, Saulo L.
波斯罗普斯阿斯佩尔和波斯罗普斯阿托克斯是厄瓜多尔重要的毒蛇，是造成大多数蛇毒事故的罪魁祸首，在过去，这些事故是用国家多药抗蛇毒血清治疗的。多年来，毒液池被收集起来，并在实验室的室温下储存。考虑到干燥样品保留其生物学效应和酶活性的争议，我们研究了储存多年的蛇毒的生化和毒理性质。用高效液相色谱和电泳法分析的历史毒液的蛋白质组图谱非常相似。新鲜批次的毒液比储存多年的毒液更具致命性，就像这些样本的初始和当前LD50值发生变化一样。部分毒液池的肌毒活性和出血活性有显著差异，而水肿性活性无显著差异。酶活性测定显示，偶氮酶和磷脂酶A(2)的蛋白分解活性存在差异，而凝血酶样丝氨酸蛋白酶活性的差异很小。蛋白质组图谱的维持和某些毒理活性将这个毒物库转化为一个有价值的研究资源。然而，使用这些样品生产抗蛇毒血清不可能显著降低毒理活性，如出血活性。
Bothrops asper and Bothrops atrox are important venomous snakes from Ecuador responsible for the most of ophidic accidents, which in the past were treated with a national polyvant antivenom. For years, the venom pools were collected and stored at room temperature in a laboratory. Taking into account the controversial ability of desiccated samples to retain their biological effects and enzymatic activities, we investigated the biochemical and toxicological properties of venoms after years of storage. The proteomic profiles of historical venoms analyzed by high-performance liquid chromatography and electrophoresis are very similar. The fresh batches of venom were more lethal than those stored for years, just as the initial and current LD50 values of these samples changed. Significant differences were showed in the myotoxic and hemorrhagic activity of some venom pools, while no significant statistical differences were found for the edema activity. The enzymatic assays revealed a variation in proteolytic activity on azocasein and phospholipase A(2) activity, and low differences were reported for thrombin-like serine protease activity. The maintenance of the proteomic profile and certain toxicological activities convert this venom library in a valuable source for research purposes. Nonetheless, the significative reduction of toxicological activities, such as hemorrhagic activity not feasible using these samples for the antivenom production.