The mutagenic action of N-ethyl-N-nitrosourea in the mouse
The mutagenic action of N-ethyl-N-nitrosourea in the mouse
复制标题
DOI：
10.1007/s003350010093
复制
发表时间：
2000-07-01
期刊：
影响因子：
2.5
通讯作者：
Justice, MJ
中科院分区：
文献类型：
--
作者：
Noveroske, JK;Weber, JS;Justice, MJ
本文重点介绍 N-乙基-N-亚硝基脲的诱变作用。 N-乙基-N-亚硝基脲 (ENU) 已成为哺乳动物遗传学中最强大的工具之一。它具有在小鼠中测试的所有种系诱变剂中最高的突变率，允许表型驱动的方法分离任何感兴趣基因的突变。它能够在体内产生单碱基对突变，从而可以详细分析基因的正常功能和突变时的生理后果，使其成为人类疾病建模的理想选择。
This article focuses on mutagenic action of N-ethyl-N-nitrosourea. N-ethyl-N-nitrosourea (ENU) has become one of the most powerful tools in mammalian genetics. It has the highest mutation rate of any germline mutagen tested in the mouse, allowing pheno type-driven approaches to isolate mutations in any gene of interest. Its ability to produce single base pair mutations in vivo allows for a detailed analysis of a gene's normal functions and the physiological consequences when mutated, making it ideal for modeling human diseases.