A gene expression map of shoot domains reveals regulatory mechanisms
A gene expression map of shoot domains reveals regulatory mechanisms
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茎结构域的基因表达图揭示了调控机制
DOI：
10.1038/s41467-018-08083-z
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发表时间：
2019-01-11
影响因子：
16.6
通讯作者：
Jiao, Yuling
中科院分区：
文献类型：
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作者：
Tian, Caihuan;Wang, Ying;Jiao, Yuling
基因调控网络通过结构域特异性基因表达控制发育。在种子植物中，位于茎顶端分生组织（SAM）中的自我更新干细胞从SAM外围区产生叶片。引发后，叶发展极性模式，形成平面形状。在这里，我们比较SAM和叶结构域之间的翻译RNA。使用翻译核糖体亲和纯化和RNA测序来量化靶结构域中的基因表达，我们生成了一个结构域特异性的翻译组图谱，覆盖了代表性的营养阶段SAM和叶结构域。我们讨论了这些领域的预测细胞功能，并提供证据，圆顶看似不相关的领域，利用共同的监管模块。实验跟踪表明，兔耳和花葵转录因子在腋芽分生组织的发生中起作用。该数据集为进一步研究芽发育和对内部和环境信号的响应提供了社区资源。
Gene regulatory networks control development via domain-specific gene expression. In seed plants, self-renewing stem cells located in the shoot apical meristem (SAM) produce leaves from the SAM peripheral zone. After initiation, leaves develop polarity patterns to form a planar shape. Here we compare translating RNAs among SAM and leaf domains. Using translating ribosome affinity purification and RNA sequencing to quantify gene expression in target domains, we generate a domain-specific translatome map covering representative vegetative stage SAM and leaf domains. We discuss the predicted cellular functions of these domains and provide evidence that dome seemingly unrelated domains, utilize common regulatory modules. Experimental follow up shows that theRABBIT EARSandHANABA TARANUtranscription factors have roles in axillary meristem initiation. This dataset provides a community resource for further study of shoot development and response to internal and environmental signals.