Expression of DJ-1 in the adult mouse CNS
Expression of DJ-1 in the adult mouse CNS
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DOI：
10.1016/j.brainres.2005.02.006
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发表时间：
2005-04-11
期刊：
影响因子：
2.9
通讯作者：
Stichel, CC
中科院分区：
文献类型：
--
作者：
Badera, V;Zhu, XR;Stichel, CC
DJ-1的隐性遗传突变最近被认为与帕金森氏症的家族优势有关。然而，DJ-1的区域和细胞表达在很大程度上尚不清楚。在本研究中，我们绘制了DJ-1的转录本和蛋白质的分布在成年小鼠中枢神经系统的非放射性原位杂交和免疫组织化学。免疫组织化学分析，我们提出了它的抗GST-小鼠DJ-1融合蛋白的多克隆抗体。DJ-1转录本和蛋白质在所有CNS区域中强烈且均匀地表达。共定位实验揭示了在不同神经递质表型的神经元和所有胶质细胞类型中的表达，如星形胶质细胞、小胶质细胞和少突胶质细胞。DJ-1蛋白弥散分布于索马胞体和突起近端的胞质中，而细胞核则无分布。DJ-1在神经元和子代细胞中的高表达，不局限于单个功能系统或任何解剖区域，支持细胞生物学中的基本生理作用的观点。(c)2005 Elsevier B. V.保留所有权利。
Recessively inherited mutations in DJ-1 have recently been linked to familial fortes of parkinsonism. However, the regional and cellular expression of DJ-1 is largely unknown. In the present study, we mapped the distribution of DJ-1 transcript and protein by non-radioactive in situ hybridization and immunohistochemistry in adult mouse CNS. For immunohistochemical analysis, we raised it polyclonal antiserum against GST-mouse DJ-1 fusion protein. DJ-1 transcript and protein were strongly and homogenously expressed in all CNS regions. Colocalization experiments revealed expression in neurons of different neurotransmitter phenotypes and in all glial cell type, such its astrocytes, microglia and oligodendrocytes. DJ-1 protein was diffusely distributed throughout the cytoplasma of the soma and the proximal parts of the processes, while the nuclei were always spared. The high expression of DJ-1 in neuronal and filial cells, that is not confined to a single functional system or any anatomical area, supports the view of a basic physiological role in cell biology. (c) 2005 Elsevier B.V. All rights reserved.