Generation of Induced Pluripotent Stem Cell Lines from Tibetan Miniature Pig
Generation of Induced Pluripotent Stem Cell Lines from Tibetan Miniature Pig
复制标题
西藏小型猪诱导多能干细胞系的产生
DOI：
10.1074/jbc.m109.008938
复制
发表时间：
2009-06-26
影响因子：
4.8
通讯作者：
Pei, Duanqing
中科院分区：
文献类型：
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作者：
Esteban, Miguel A.;Xu, Jianyong;Pei, Duanqing
诱导多能干细胞（iPS）技术似乎是从任何给定的哺乳动物物种产生多能干细胞的一般策略。到目前为止，已经报道了小鼠、人、大鼠和猴的iPS细胞。这四个物种还建立了胚胎干细胞（ESC）系，作为多能性比较的金标准。已经尝试通过各种手段产生猪ESC，但没有成功。在这里，我们报告了成功的一代多能干细胞从成纤维细胞分离的西藏小型猪使用修改的iPS协议。所得到的iPS细胞系比来自其他物种的细胞更接近于人ESC，具有正常的核型，碱性磷酸酶染色阳性，表达高水平的ESC样标记物（Nanog，Rex 1，Lin28和SSEA 4），并且可以分化成由三个胚层组成的畸胎瘤。由于猪的生理学与人类非常相似，本文报道的iPS细胞为研究某些人类疾病或评估iPS在大型动物模型中的治疗应用提供了一个有吸引力的模型。
Induced pluripotent stem cell (iPS) technology appears to be a general strategy to generate pluripotent stem cells from any given mammalian species. So far, iPS cells have been reported for mouse, human, rat, and monkey. These four species have also established embryonic stem cell (ESC) lines that serve as the gold standard for pluripotency comparisons. Attempts have been made to generate porcine ESC by various means without success. Here we report the successful generation of pluripotent stem cells from fibroblasts isolated from the Tibetan miniature pig using a modified iPS protocol. The resulting iPS cell lines more closely resemble human ESC than cells from other species, have normal karyotype, stain positive for alkaline phosphatase, express high levels of ESC-like markers (Nanog, Rex1, Lin28, and SSEA4), and can differentiate into teratomas composed of the three germ layers. Because porcine physiology closely resembles human, the iPS cells reported here provide an attractive model to study certain human diseases or assess therapeutic applications of iPS in a large animal model.