Proteome of amyloplasts isolated from developing wheat endosperm presents evidence of broad metabolic capability
Proteome of amyloplasts isolated from developing wheat endosperm presents evidence of broad metabolic capability
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DOI：
10.1093/jxb/erj156
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发表时间：
2006-04-01
影响因子：
6.9
通讯作者：
Hurkman, WJ
中科院分区：
文献类型：
--
作者：
Balmer, Y;Vensel, WH;Hurkman, WJ
与叶绿体相比，我们对造粉体（在异养植物组织中合成和储存淀粉的细胞器）的认识还处于形成阶段。虽然近年来我们对它们的主要功能，即淀粉的生物合成和降解的理解急剧增加，但对这些细胞器中发生的其他生物化学过程知之甚少。为了帮助填补这一空白，蛋白质组学分析淀粉体分离小麦种子（开花后10天）的淀粉胚乳已进行。这项研究已经确定了289种蛋白质，它们在一系列过程中发挥作用，包括碳水化合物代谢、细胞骨架/质体分裂、能量学、氮和硫代谢、核酸相关反应、各种构建模块的合成、蛋白质相关反应、运输、信号传导、应激和各种其他活动。12%的蛋白质的功能是未知的。这些结果突出了造粉体作为淀粉储存细胞器的作用，它满足了母体组织的生物合成需求。与最近的蛋白质组学分析的整个胚乳相比，目前的研究表明，在蛋白质组学研究中使用分离的细胞器的优势。
By contrast to chloroplasts, our knowledge of amyloplasts-organelles that synthesize and store starch in heterotrophic plant tissues-is in a formative stage. While our understanding of what is considered their primary function, i.e. the biosynthesis and degradation of starch, has increased dramatically in recent years, relatively little is known about other biochemical processes taking place in these organelles. To help fill this gap, a proteomic analysis of amyloplasts isolated from the starchy endosperm of wheat seeds (10 d post-anthesis) has been conducted. The study has led to the identification of 289 proteins that function in a range of processes, including carbohydrate metabolism, cytoskeleton/plastid division, energetics, nitrogen and sulphur metabolism, nucleic acid-related reactions, synthesis of various building blocks, protein-related reactions, transport, signalling, stress, and a variety of other activities grouped under 'miscellaneous'. The function of 12% of the proteins was unknown. The results highlight the role of the amyloplast as a starch-storing organelle that fulfills a spectrum of biosynthetic needs of the parent tissue. When compared with a recent proteomic analysis of whole endosperm, the current study demonstrates the advantage of using isolated organelles in proteomic studies.