Noise in gene expression determines cell fate in Bacillus subtilis
Noise in gene expression determines cell fate in Bacillus subtilis
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DOI：
10.1126/science.1140818
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发表时间：
2007-07-27
期刊：
影响因子：
56.9
通讯作者：
Dubnau, David
中科院分区：
文献类型：
--
作者：
Maamar, Hedia;Raj, Arjun;Dubnau, David
等基因群体中基因表达的随机细胞对细胞变异会导致基因表达的替代状态之间的过渡。关于自然系统中的这些变化（噪声）如何影响此类过渡，知之甚少。在枯草芽孢杆菌中，comk中的噪声是调节DNA摄取能力的蛋白质，被认为会导致细胞过渡到表达DNA摄取蛋白的基因的合理状态。我们证明，昏迷表达中的噪声会为能力选择细胞，并且该噪声的实验降低会减少胜任的细胞数量。我们还表明，通过减少康克转录的降低，过渡受到了时间的限制。这些结果说明了这种随机转变如何在自然系统中调节，并表明噪声特征受到进化力。
Random cell-to-cell variations in gene expression within an isogenic population can lead to transitions between alternative states of gene expression. Little is known about how these variations ( noise) in natural systems affect such transitions. In Bacillus subtilis, noise in ComK, the protein that regulates competence for DNA uptake, is thought to cause cells to transition to the competent state in which genes encoding DNA uptake proteins are expressed. We demonstrate that noise in comK expression selects cells for competence and that experimental reduction of this noise decreases the number of competent cells. We also show that transitions are limited temporally by a reduction in comK transcription. These results illustrate how such stochastic transitions are regulated in a natural system and suggest that noise characteristics are subject to evolutionary forces.