Fluorescence spectroscopy in a shoebox
Fluorescence spectroscopy in a shoebox
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DOI：
10.1021/ed084p1308
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发表时间：
2007-08-01
影响因子：
3
通讯作者：
Wahab, M. Farooq
中科院分区：
文献类型：
--
作者：
Wahab, M. Farooq
本文介绍了一种结构简单、价格低廉的荧光计。它利用手电筒或太阳光源，荧光笔墨水，一碗水与镜子作为分散元素，和彩色玻璃纸作为过滤器。人眼被用作探测器。该装置用于演示与荧光光谱学相关的重要概念。使用来自荧光笔标记的墨水，可以证明光散射和荧光发射之间的差异、对强光源的需要、斯托克斯位移现象、滤光片的选择、激发源和发射检测器的优选几何形状以及可以通过荧光测量实现的低检测限。通过反射光盘发出的荧光，可以看出分子发出的光不是单色的。此外，使用由DVD或CD制成的光栅来构造分光荧光计。鞋盒荧光计和分光荧光计可以作为有用的教学辅助工具，在那些地方没有商业仪器，它避免了现代仪器的黑箱问题。
This article describes construction of a simple, inexpensive fluorometer. It utilizes a flashlight or sunlight source, highlighter marker ink, bowl of water with mirror as dispersing element, and colored cellophane sheets as filters. The human eye is used as a detector. This apparatus is used to demonstrate important concepts related to fluorescence spectroscopy. Using ink from a highlighter marker, one can demonstrate the difference between light scattering and fluorescence emission, the need for an intense light source, phenomenon of the Stokes shift, the choice of filters, the preferred geometry of excitation source and emission detector, and the low detection limits that can be achieved by fluorescence measurements. By reflecting the fluorescence emission from a compact disk, it can be seen that the light emitted by molecules is not monochromatic. Furthermore, a spectrofluorometer is constructed using gratings made from a DVD or a CD. The shoebox fluorometer and spectrofluorometer can serve as useful teaching aids in places where commercial instruments are not available, and it avoids the black box problem of modern instruments.