Rat lung lavage surfactant enhances bacterial phagocytosis and intracellular killing by alveolar macrophages.
Rat lung lavage surfactant enhances bacterial phagocytosis and intracellular killing by alveolar macrophages.
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大鼠肺灌洗表面活性剂增强肺泡巨噬细胞的细菌吞噬作用和细胞内杀伤作用。
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发表时间：
1984
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通讯作者：
D. Klass
中科院分区：
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作者：
S. O’Neill;E. Lesperance;D. Klass
本文研究了大鼠肺灌洗液表面活性剂（SAM）对大鼠肺泡巨噬细胞（AM）吞噬和杀伤金黄色葡萄球菌的作用。通过密度梯度离心分离和纯化SAM。它高度富含二饱和磷脂酰胆碱，并且总是将清洁盐水溶液的表面张力在37摄氏度下降低到小于10达因/厘米。我们使用放射性测定来测量吞噬作用作为AM对14 C苯丙氨酸标记的葡萄球菌的摄取，以及细胞内杀伤作用作为来自裂解AM的活葡萄球菌对3 H胸苷的掺入。当葡萄球菌与100至300微克SAM孵育时，吞噬作用和细胞内杀伤效率均增强。SAM组和对照组细胞内葡萄球菌/AM的平均数量分别为24.3 +/- 3.8和18.3 +/- 3.0（p小于0.001）。SAM组和对照组的平均细胞内杀伤效率分别为59.6 +/- 6.76%和39.7 +/- 7.17%（p <0.001）。先前的研究表明，未纯化的大鼠肺灌洗液增强AM对葡萄球菌的细胞内杀伤。我们的研究结果表明，SAM可能是肺灌洗液中增强细胞内杀伤的活性成分。此外，在我们的测试系统中，SAM增强AM对葡萄球菌的吞噬作用。
We have tested the effect of a preparation of rat lung lavage surfactant (SAM) on phagocytosis and intracellular killing of Staphylococcus aureus by rat alveolar macrophages (AMs). The SAM was isolated and purified by density gradient centrifugation. It was highly enriched in disaturated phosphatidylcholine, and invariably lowered the surface tension of a clean saline solution to less than 10 dynes/cm at 37 degrees C. We used a radiometric assay to measure phagocytosis as uptake by AMs of 14C phenylalanine labelled staphylococci, and intracellular killing as incorporation of 3H thymidine by viable staphylococci from the lysed AMs. When staphylococci were incubated with 100 to 300 micrograms of SAM, both phagocytosis and intracellular killing efficiency were enhanced. The mean numbers of intracellular staphylococci/AM were 24.3 +/- 3.8 and 18.3 +/- 3.0 in the SAM and control groups, respectively (p less than 0.001). The mean intracellular killing efficiency was 59.6 +/- 6.76% and 39.7 +/- 7.17% in the SAM and control groups, respectively (p less than 0.001). Previous studies have shown that unpurified rat lung lavage fluid enhances intracellular killing of staphylococci by AMs. Our results suggest that SAM may be the active principle in lung lavage fluid that enhances intracellular killing. In addition, in our test system, SAM enhanced staphylococcal phagocytosis by AMs.