Short-Flagella Mutants of Chlamydomonas reinhardtii.
Short-Flagella Mutants of Chlamydomonas reinhardtii.
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DOI：
10.1093/genetics/115.4.685
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发表时间：
1987-04
期刊：
影响因子：
3.3
通讯作者：
Michael R. Kuchka;Jonathan W. Jarvik
中科院分区：
文献类型：
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作者：
Michael R. Kuchka;Jonathan W. Jarvik
通过筛选慢泳者的诱变衣原体克隆，分离出六个短鞭毛突变体。这六个突变体鉴定出三个不连锁的孟德尔基因，其中三个突变在基因shf-1中，两个在shf-2中，一个在shf-3中。shf-1和shf-2已分别定位于染色体VI和I。两个shf-1突变体具有温度敏感的鞭毛组装表型，一个shf-2突变体具有冷敏感表型。构建SHF SHF双突变体;根据存在的等位基因，它们显示无鞭毛或短鞭毛表型。分离出shf-1和shf-2突变体的表型回复体，发现它们中的某些带有基因外抑制子，有些是显性的，有些是隐性的。我们怀疑shf突变影响了一个特定的鞭毛大小控制系统的组成部分，该系统的存在已被各种生理实验所证实。
Six short-flagella mutants were isolated by screening clones of mutagenized Chlamydomonas for slow swimmers. The six mutants identify three unlinked Mendelian genes, with three mutations in gene shf-1, two in shf-2 and one in shf-3. shf-1 and shf-2 have been mapped to chromosomes VI and I, respectively. Two of the shf-1 mutations have temperature-sensitive flagellar-assembly phenotypes, and one shf-2 mutant has a cold-sensitive phenotype. shf shf double mutants were constructed; depending on the alleles present they showed either flagellaless or short-flagella phenotypes. Phenotypic revertants of shf-1 and shf-2 mutants were isolated, and certain of them were found to carry extragenic suppressors, some dominant and some recessive. We suspect that the shf mutations affect components of a specific flagellar size-control system, the existence of which has been suggested by a variety of physiological experiments.