Gene expression in women conceiving spontaneously over the age of 45 years
Gene expression in women conceiving spontaneously over the age of 45 years
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DOI：
10.1016/j.fertnstert.2007.06.058
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发表时间：
2008-06-01
影响因子：
6.7
通讯作者：
Laufer, Neri
中科院分区：
文献类型：
--
作者：
Gielchinsky, Yuval;Bogoch, Yoel;Laufer, Neri
目的：研究45岁以后自然受孕妇女的基因表达谱。设计：互补DNA微阵列分析表达谱。单位：大学院系。患者（S）：8名45岁或以上的妇女（研究组）自发怀孕和6个月后，他们的最后一次分娩进行了比较，6名妇女年龄45岁干预（S）：从每个妇女收集血液样品，用于从外周血单核细胞（PBMC）分离RNA。主要结果测量（g）：通过使用覆盖约15，000个鉴定的基因的Affytechnic DNA微阵列分析来确定从每个妇女分离的PBMC的表达谱。全球基因表达的微阵列显示671个基因在研究组和对照组之间显示出统计学显著的差异表达：383个基因过表达，288个基因表达不足。定义这些基因的最重要的功能组是：细胞凋亡，泛素化和能量产生。多达60个基因也参与了卵巢physiology. Conclusions（s）：这些观察结果表明，延长生育能力与一个独特的能力，丰富的细胞过程，导致卵巢衰老延迟。（Fertil Steril（R）2008;89：1641-50. (c)2008年，美国生殖医学会（American Society for Reproductive Medicine）。
Objective: To examine gene expression profiles of women conceiving spontaneously after the age of 45.Design: Expression profiling by complementary DNA microarray analysis.Setting: University departments.Patient(S): Eight women 45 years or older (study group) who conceived spontaneously and were 6 months after their last delivery were compared with six women aged 45 years old (control group) who had their last delivery before the age of 30.Intervention(S): Blood samples were collected from each woman for RNA isolation from peripheral blood mononuclear cells (PBMC).Main Outcome Measure(g): Expression profiles of PBMC isolated from each woman were determined by using Affymetrix DNA microarray analysis covering about 15,000 identified genes.Result(s): Microarray of global gene expression revealed 671 genes that showed statistically significant differential expression between the study and control groups: 383 genes were overexpressed and 288 were underexpressed. The most significant functional groups defining these genes were: apoptosis, ubiquitination, and energy production. As many as 60 genes also participated in ovarian physiology.Conclusion(s): These observations suggest that extended fertility is associated with a unique ability to enrich cellular processes, leading to delayed ovarian senescence. (Fertil Steril (R) 2008;89:1641-50. (c) 2008 by American Society for Reproductive Medicine.).