Determination of dopamine, serotonin, and their metabolites in pediatric cerebrospinal fluid by isocratic high performance liquid chromatography coupled with electrochemical detection
Determination of dopamine, serotonin, and their metabolites in pediatric cerebrospinal fluid by isocratic high performance liquid chromatography coupled with electrochemical detection
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DOI：
10.1002/bmc.1338
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发表时间：
2010-06-01
影响因子：
1.8
通讯作者：
Stewart, Clinton F.
中科院分区：
文献类型：
--
作者：
Hubbard, K. Elaine;Wells, Amy;Stewart, Clinton F.
快速测定脑脊液中多巴胺（DA）、二羟吲哚苯乙酸、高香草酸、5-羟色胺（5-HT）和5-羟吲哚乙酸的方法尚未见报道。因此，开发了一种快速，灵敏，特异的HPLC方法，采用电化学检测。在冷冻前将CSF与抗氧化剂溶液混合，以防止神经递质降解。在ESA MD-150 x 3.2 mm色谱柱上，流速为0.37 mL/min，乙腈-水（5：95，v/v）移动的相，含75 mm磷酸二氢钠缓冲液、0.5 mm EDTA、0.81 mm辛基磺酸钠和5%四氢呋喃。最佳电位设置为：保卫细胞+325 mV，E1 - 100 mV，E2 + 300 mV。日内和日间精密度为
A method to rapidly measure dopamine (DA), dihydroxyindolphenylacetic acid, homovanillic acid, serotonin (5-HT) and 5-hydroxyindoleacetic acid concentrations in cerebrospinal fluid (CSF) has not yet been reported. A rapid, sensitive, and specific HPLC method was therefore developed using electrochemical detection. CSF was mixed with an antioxidant solution prior to freezing to prevent neurotransmitter degradation. Separation of the five analytes was obtained on an ESA MD-150 x 3.2 mm column with a flow rate of 0.37 mL/min and an acetonitrile-aqueous (5:95, v/v) mobile phase with 75 mm monobasic sodium phosphate buffer, 0.5 mm EDTA, 0.81 mm sodium octylsulfonate and 5% tetrahydrofuran. The optimal electrical potential settings were: guard cell +325 mV, E1 -100 mV and E2 +300 mV. Within-day and between-day precisions were